摘要
为从中药茵陈中分离内生细菌,筛选具有抑菌活性的菌株,分析活性成分。研究采用7种不同的培养基分离菌株,对分离到的菌株进行形态学和分子生物学鉴定,并对发酵产物进行抑菌活性测定,并利用分光光度法和高效液相色谱法分析抑菌活性较高的菌株发酵液活性成分。结果表明从茵陈新鲜组织中共分离到52株内生细菌,分属于9个种,芽孢杆菌为优势菌群。筛选得到具有抑菌活性的菌株17株,其中NA-3、BPA-24和KMB-323株菌活性较强,NA-3和KMB-32的发酵液中齐墩果酸含量较高。本研究通过分离纯化茵陈内生细菌,明确了茵陈内生细菌的菌群类别,获得了产齐墩果酸的两个菌株,为微生物发酵生产活性物质提供了素材。
This research aimed to isolate endophytic bacteria from Artemisia capillaris and to screen antibiotic bacteria strains.7 different kinds of culture medium were used.The isolated strains were identified by means of morphology and molecular biology,the antibacterial activity of fermentation products was tested by 96-well plate method and active ingredients in fermentation products were analyzed by spectrophotometry and high performance liquid chromatography.The results showed that 52 endophytic bacteria strains were obtained from Artemisia capillaris and were affiliated to 9 genera,bacillus was the dominant genus.17 strains showed antimicrobial activity on the indicator bacteria,strains of NA-3,BPA-24 and KMB-32 performed strong bacteriostatic activity,and high content of oleanolic acid was detected in the fermentation products of stains NA-3 and KMB-32.The study clears about the variety of endophytic bacteria of Artemisia capillaris,two strains were obtained for producing oleanolic acid which provides material endophytes for further research of active substances.
作者
鲍永毅
陈晓
牛明福
李梦圆
付应建
刘瑞文
郑少亭
BAO Yong-yi;CHEN Xiao;NIU Ming-fu;LI Meng-yuan;FU Ying-jian;LIU Rui-wen;ZHENG Shao-ting(Key laborary of Microbial resources Development and Utilization,College of food and bioengineering,He’nan University of Science and Technology,Luoyang 471023,China;National veterinary medicine engineering technology research center,Luoyang 471003,China)
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2019年第11期1919-1927,共9页
Natural Product Research and Development
基金
河南省重点科技攻关项目(172102310327)
河南省产学研项目(142107000080)
河南科技大学大学生科研训练计划(SRTP)(2018160)
关键词
茵陈内生细菌
分离鉴定
抑菌活性
高效液相色谱
齐墩果酸
endophytic bacteria
Artemisia capillaris
isolation and identification
antibacterial activity
high performance liquid chromatography(HPLC)
oleanolic acid
作者简介
共同第一作者:鲍永毅;共同第一作者:陈晓;通信作者:牛明福,E-mail:mingfuniu@163.com。
