摘要
目的:研究淫羊藿女贞子配伍对绝经后骨质疏松症(PMOP)大鼠雌激素作用的影响。方法:双侧卵巢切除术模拟PMOP大鼠模型,随机分为假手术组、模型组、淫羊藿女贞子配伍组(配伍组)、淫羊藿组、女贞子组、雷洛昔芬组(阳性药组),每组8只。术后1周开始给予相应的药物治疗12周,酶免疫法检测血清中雌二醇(E2)含量,自动生化法检测肝肾功能指标丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、肌酐(CR)和尿酸(UA)含量,免疫荧光、Western Blotting和实时荧光定量PCR技术检测骨组织雌激素受体(ER)两种亚型的蛋白及mRNA表达,苏木精-伊红染色检测骨组织病理学改变。结果:与假手术组比较,PMOP大鼠血清E2含量和骨组织ERα和ERβmRNA及蛋白表达均显著降低,差异有统计学意义(P<0.01);肝肾功能指标均显著升高,差异有统计学意义(P<0.01);同时出现典型的骨质疏松病理改变。与模型组比较,配伍组能显著升高血清E2含量,差异有统计学意义(P<0.01);降低肝肾功能指标的水平,差异有统计学意义(P<0.05或P<0.01);上调骨组织中ERα及ERβ的蛋白及mRNA表达,差异有统计学意义(P<0.01);显著改善骨质疏松的病理改变。结论:淫羊藿女贞子配伍防治PMOP的作用与显著上调雌激素及雌激素受体的表达有关。
Objective:To investigate the effects of the combination of herba epimedii and fructus ligustri lucidi on estrogen and estrogen receptor(ER)in ovariectomy(OVX)-induced osteoporosis of rats.Methods:PMOP rat model was duplicated by bilateral ovariotomy.Rats were randomly divided into 6 groups,including sham group,model group,Herba Epimedii group,fructus ligustri lucidi group,combination group and raloxifene group.Estradiol(E2),ALT(alanine aminotransferase),AST(aspartic transaminase),CR(creatinine) and UA(uric acid) in blood,the mRNA and protein expression of estrogen receptor(ER)in bone,and bone histomorphometry analysis were detected.Results:Compared with the sham group,serum E2 level and the mRNA and protein expression of ER in bone were reduced(P<0.01),while serum ALT,AST,CR and UA were significantly increased(P<0.01);pathological changes of osteoporosis were detected in the model group.Compared with the model group,herba epimedii and fructus ligustri lucidi in combination could significantly increased the level of serum E2,reduced serum ALT,AST,CR and UA,up-regulated the expression of mRNA and protein of ERα and ERβ in bone tissue,and improving the pathological changes of osteoporosis.Conclusion:Herba epimedii and fructus ligustri lucidi in combination has the anti-osteoporosis on PMOP through the up-regulation of estrogen and ER.
作者
李晓曦
陈宇恒
唐秀凤
高莹莹
于萍
马紫童
刘仁慧
LI Xiaoxi;CHEN Yuheng;TANG Xiufeng;GAO Yingying;YU Ping;MA Zitong;LIU Renhui(School of Traditional Chinese Medicine,Capital Medical University,Beijing 100069,China)
出处
《中国中医骨伤科杂志》
CAS
2019年第9期1-6,10,共7页
Chinese Journal of Traditional Medical Traumatology & Orthopedics
基金
国家自然科学基金资助项目(81673993)
国家中医药管理局全国名老中医药专家传承工作室建设项目
关键词
骨质疏松症
淫羊藿/女贞子
雌激素
雌激素受体
osteoporosis
herba epimedii/fructus ligustri lucidi
estrogen
estrogen receptor
作者简介
通信作者:刘仁慧,E-mail:liurenhui995@163.com。
