摘要
目的:探究小鼠卵巢颗粒细胞中微管相关蛋白的表达及功能定位。方法:选取雌性小鼠发生6周成年(成年组,6只)与未成年(未成年组,6只),培养卵巢颗粒细胞,将加入促卵泡刺激素(FSH)培养的颗粒细胞作为FSH组,不加FSH培养的颗粒细胞作为非FSH组。免疫组化染色法观察颗粒细胞中微管相关蛋白tau表达,将tau融合蛋白转染到颗粒细胞上,荧光显微镜下观察tau蛋白在颗粒细胞中的定位。结果:小鼠卵巢颗粒细胞tau蛋白表达阳性数成年组高于未成年组,FSH组高于非FSH组(P<0.05);未成年小鼠中FSH组阳性数高于非FSH组(P<0.05)。成年组小鼠卵巢颗粒细胞中tau蛋白聚集于细胞核,呈放射状。结论:微管相关蛋白tau在成年小鼠卵巢颗粒细胞中高表达,其定位主要集中于细胞核,影响颗粒细胞增殖分化,通过观察tau蛋白表达及定位,可反映微管形成及形态变化。
Objective:To investigate the expression and function localization of microtubule associated protein tau in mouse ovarian granulosa cells.Methods:12 female mice were selected,which included 6 adult mice and 6 juvenile mice,and their ovarian granulosa cells were collected and cultured.The granulosa cells of these mice were cultured with or without FSH,and immunohistochemical staining was used to observe the expression of microtubule associated protein tau in the granulosa cells.Then Tau fusion protein was transfected into the granulosa cells,and the localization of tau in the granulosa cells was observed by fluorescence microscopy.Results:The positive expression of tau protein in the ovarian granulosa cells of the adult was significant higher than that of juvenile mice.Among the adult mouse,the positive expression of tau protein in the granulosa cells cultured with FSH was significant higher than that of cells cultured without FSH(P<0.05).Among the juvenile mouse,the positive expression of tau protein in ovarian granulosa cells of cultured with FSH was significant higher than that of ovarian granulosa cells cultured without FSH(P<0.05).Tau protein of ovarian granulosa cells of adult mice was aggregated in nucleus and was radiated.Conclusion:The microtubule associated protein Tau can be highly expressed in ovarian granulosa cells of adult mouse,and its localization is mainly concentrated in the nucleus.The expression and localization of tau can reflect the formation and morphological changes of microtubules,which affect the proliferation and differentiation of microtubule of granulosa cells.
作者
吕辉
杨晓霞
韦启鹏
LV Hui;YANG Xiaoxia;WEI Qipeng(Affiliated Hospital of Hubei University of Arts and Sciences College,Xiangyang City Central Hospital,Hubei Province,441021)
出处
《中国计划生育学杂志》
2019年第9期1133-1137,共5页
Chinese Journal of Family Planning
基金
襄阳市科技局医疗卫生指导性科技计划项目(2017ZD06)
关键词
卵巢颗粒细胞
微管蛋白tau
促卵泡刺激素
小鼠
Ovarian granulosa cell
Microtubule associated protein tau
Follicle stimulating hormone
Mouse
作者简介
通信作者:韦启鹏,zlw156113405@163.com。
