摘要
辣木富含维生素、蛋白质和各种微量元素,具很好的保健作用。本试验以辣木种子为外植体,研究植物生长调节剂对辣木不定芽诱导、增殖以及生根的影响。结果表明:适合辣木不定芽诱导的培养基为MS+1.0 mg/L 6-BA+0.2 mg/L NAA;适合辣木不定芽增殖的培养基为MS+0.5 mg/L 6-BA+0.2 mg/L NAA;辣木不定芽生根的培养基以1/2 MS+0.3 mg/L IBA+0.2 mg/L NAA+1 g/L活性炭为佳,根的生长都优于其他培养基。在生根培养基中,NAA和IBA两种植物生长调节剂配合使用优于NAA、IBA单独使用。本研究为辣木组织培养技术的发展提供了研究参考。
Moringa is rich in vitamin, protein and microelements and have great health benefits. In this research,using the seeds of the moringa as explants, the effects of plant growth regulator on adventitious bud induction,proliferation and rhizogenesis were studied. The results showed that the optimum condition for adventitious buds induction was MS supplemented with 1.0 mg/L 6-BA and 0.2 mg/L NAA; MS supplemented with 0.5 mg/L 6-BA and 0.2 mg/L NAA was ideal for adventitious buds proliferation; 1/2 MS supplemented with 0.3 mg/L IBA, 0.2 m/L NAA and 1.0 g/L AC was the optimum medium for root induction and growth in adventitious buds. Roots are growing better than other media. Adding in the rooting medium, NAA and IBA was better than NAA or IBA,respectively. This research would lay a foundation for tissue culture study on Moringa oleifera Lam.
出处
《分子植物育种》
CAS
CSCD
北大核心
2016年第11期3154-3158,共5页
Molecular Plant Breeding
基金
农业部热作技术试验示范项目(15162130106232013-4)
福建省省属公益类科研专项(2014R1014-14)
福建省现代农业技术支撑工程(辣木高效栽培和加工技术研究与示范)
福建省农业科学院院导师制青年基金
青年人才创新基金项目(2014QB-30)共同资助
关键词
辣木
生长调节剂
组培
快繁
Moringa oleifera Lam.
Hormones
Tissue culture
Rapid propagation
