摘要
目的 研究 1alpha ,2 5 (OH) 2 维生素D3 (1alpha ,2 5 (OH) 2 vitaminD3 ,1α ,2 5 (OH) 2 vitD3 )对人牙周膜细胞骨保护素 (osteoprotegerin ,OPG)及破骨细胞分化因子 (receptoractivatornuclearfactorkappaBligand ,RANKL)表达的影响。方法 组织块法培养人牙周膜细胞 ,1α ,2 5 (OH) 2 vitD3 诱导 0、2、4、6天 ,半定量逆转录聚合酶链反应(reversetranscription -polymerasechainreaction ,RT -PCR)检测细胞OPG和RANKL在信使RNA(messengerRNA ,mRNA)水平表达的变化。结果 随着 1α ,2 5 (OH) 2 vitD3 作用时间的延长 ,人牙周膜细胞OPG的表达降低 ,RANKL的表达增高 ,OPG/RANKL比值降低并具有时间依赖性。结论 在 1α ,2 5 (OH) 2 vitD3 对人牙周膜细胞的诱导周期中 ,OPG和RANKL骨代谢通路发挥作用。这一结论为探讨 1α ,2 5 (OH) 2 vitD3
Objective To study the expression of osteoprotegerin(OPG)and receptor activator nuclear factor kappa B ligand(RANKL)in human periodontal ligament cells(hPDLCs)induced by 1alpha,25(OH) 2 vitamin D 3 (1α,25(OH) 2vitD 3).Methods HPDLCs were cultured by explant method and were induced by 1α,25(OH) 2vitD 3 for 0,2,4,6 days. Semi-quantitative reverse transcription-polymerase chain reaction(RT-PCR)examination indicated the expression of OPG and RANKL at messenger RNA(mRNA)level in hPDLCs.Results OPG mRNA expression was down-regulated, RANKL mRNA expression was up-regulated, and the ratio of OPG/RANKL was decreased by 1α,25(OH) 2vitD 3 in a time dependent manner.Conclusion The bone metabolism path of OPG and RANKL worked during the process of 1α,25(OH) 2vitD 3 inducing hPDLCs.The conclusion did the groundwork for the study on bone remodeling mechanisms of 1α,25(OH) 2vitD 3.
出处
《现代口腔医学杂志》
CAS
CSCD
2002年第6期481-484,共4页
Journal of Modern Stomatology
基金
国家自然科学基金资助项目 (编号:3 0 0 70 82 1)
北京大学医学部"985"学科建设项目
