摘要
目的探讨腹膜透析(PD)在冲击波诱导的大鼠肺损伤中的疗效及机制。方法45只成年Sprague Dawley大鼠随机分为对照组、假手术组和PD组。采用55 kg冲击波(BST-I)制造大鼠肺冲击伤。假手术组和PD组在冲击伤前行腹部置管术。PD组致伤1 h后给予2.5%腹膜透析液20 ml留腹,30 min后放出,如此反复12个循环。透析6 h后处死全部大鼠。部分损伤肺组织行HE染色评价病理形态学改变,剩余肺组织测肺含水量。收集动脉血液,血气分析仪和小动物肺功能检测仪检测肺功能。ELISA检测血清炎性因子肿瘤坏死因子α(TNF-α)、白细胞介素(IL)-1β、IL-6、单核细胞趋化蛋白1(MCP-1)的水平。结果与对照组比较,PD组肺组织肺泡结构相对完整,肺间质水肿减轻、炎性细胞浸润较少。PD组肺含水量较对照组明显降低(P<0.05)。PD组血清TNF-α、IL-1β、IL-6、MCP-1均低于对照组(均P<0.05)。PD组氧饱和度、氧分压、氧合指数、肺活量、功能残气量、最大呼气中期流率均高于对照组(均P<0.05)。结论PD通过清除肺多余水分、血清中炎性因子,从而改善冲击波诱导的大鼠急性肺损伤。
Objective To prove the efficacy of peritoneal dialysis on shock wave-induced acute lung injury of rats, and analyze its mechanisms. Methods Forty-five adult Sprague-Dawley rats were randomly divided into three groups: control group, sham operation (Sham) group and peritoneal dialysis (PD) group. Sham group and PD group did abdominal catheterization before blast injury. The 55 kg shock wave (bst-I) was used to induce lung blast injury. After one hour of blast injury, PD group was given 2.5% peritoneal dialysate 20 ml to stay abdomen, which was released 30 min posted, repeated 12 cycles. After 6 hours of peritoneal dialysis, all of the rats were sacrificed. Partial damaged tissues in lung were used to evaluate the pathomorphologic changes by HE staining, and the remnants were used to measure the lung water content. Lung function was detected by blood gas analyzer and small animal detector from the arterial blood gas. The levels of serum inflammatory factors, such as tumor necrosis factor-α(TNF-α), interleukin (IL)-1β, IL-6 and monocyte chemoattractant protein-1 (MCP-1) were tested by ELISA. Results The relative integrity of alveolar structure, interstitial edema and inflammatory cell infiltration in PD group were significantly improved than those in control group. The lung water content of PD group was significantly lower than that of control group (P<0.05). The levels of TNF-α, IL-1β, IL-6 and MCP-1 in serum of PD group were significantly lower than those in control group (all P<0.05). The blood oxygen saturation, oxygen partial pressure, oxygenation index, vital capacity, functional residual volume and maximum mid-expiratory flow rate in PD group were significantly higher than those in control group (all P<0.05). Conclusions Through reducing pulmonary edema and inflammatory factors, peritoneal dialysis can improve lung function in shock wave -induced acute lung injury of rats.
作者
胡威
陈客宏
汪晓月
杜鹃
何娅妮
Hu Wei;Chen Kehong;Wang Xiaoyue;Du Juan;He Yani(Department of Nephrology,Army Characteristic Medical Center,Chongqing 400010,China;Department of Nephrology,Army 958 Hospital,Chongqing 400020,China;Department of NO.4 Field Surgery,Army Special Medicine Center,Chongqing 400010,China)
出处
《中华肾脏病杂志》
CAS
CSCD
北大核心
2019年第9期684-689,共6页
Chinese Journal of Nephrology
基金
国家自然科学基金面上项目(81670661)
十二五军队重大项目(AWS14C003).
关键词
腹膜透析
肺损伤
细胞因子类
炎症
呼吸功能试验
Peritoneal dialysis
Lung injury
Cytokines
Inflammation
Respiratory function tests
作者简介
通信作者:何娅妮,Email:heynmail@sina.com.
