摘要
目的研究左旋多巴原料药精制母液的化学成分。方法精制母液95%乙醇提取物采用硅胶、大孔树脂D101、制备HPLC等进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构。结果从中分离得到12个化合物,分别鉴定为豆甾醇(1)、棕榈酸(2)、正三十烷醇(3)、十八酸-2,3-二羟基丙酯(4)、soyasapogrnol B(5)、硬脂酸(6)、亚油酸(7)、5α,8α-环二氧-(22E,24R)-麦角甾-6,22-二烯-3β-醇(8)、原儿茶酸(9)、6,7-dihydroxy-1-methyl-1,2,3,4-tetrahydro-isoquinoline-3-carboylic acid(10)、6,7-dihydroxy-1-benzyl-1,2,3,4-tetrahydro-isoquinoline-3-carboylic acid(11)、苯丙氨酸(12)。结论所有化合物均为首次从该精制母液中分离得到。
AIM To study the chemical constituents of purification solution from raw material of levodopa. METHODS The 95% ethanol extract of purification solution was isolated and purified by silica,macroporous resin D101 and preparative HPLC,then the structures of obtained compounds were identified by physicochemical properties and spectral data.RESULTS Twelve compounds were isolated and identified as stigmasterol(1), palmitic acid(2), n-triacontanol(3), propyl 2,3-dihydroxy octadecanoate(4), soyasapogrnol B(5), octadecanoic acid(6), linoleic acid(7), 5α,8α-epi-dioxyergosta-(22E,24R)-6,22-dien-3β-ol(8), protocatechuic acid(9), 6,7-dihydroxy-1-methyl-1,2,3,4-tetrahydro-isoquinoline-3-carboylic acid(10), 6,7-dihydroxy-1-benzyl-1,2,3,4-tetrahydro-isoquinoline-3-carboylic acid(11), phenylalanine(12). CONCLUSION All the compounds are isolated from the purification solution for the first time.
作者
黄艳
赵小超
李学坚
卢文杰
苏彩珍
柴玲
刘布鸣
HUANG Yan;ZHAO Xiao-chao;LI Xue-jian;LU Wen-jie;SU Cai-zhen;CHAI Ling;LIU Bu-ming(Guangxi Key Laboratory of Traditional Chinese Medicine Quality Standards,Guangxi Institute of Chinese Medical & Pharamaceutical Sciences,Nan- ning 530022,China;Scientific Research Center,Guangxi University of Chinese Medicine,Nanning 530222,China;Guangxi Bonres Pharmaceu- ticals Co.,Ltd.,Baise 531599,China)
出处
《中成药》
CAS
CSCD
北大核心
2019年第9期2122-2126,共5页
Chinese Traditional Patent Medicine
基金
广西中药质量标准研究重点实验室开放课题基金项目(桂中重开201507)
关键词
左旋多巴
精制母液
化学成分
分离鉴定
levodopa
purification solution
chemical constituents
isolation and identification
作者简介
黄艳(1987—),女,副研究员,研究方向为药物分析。Tel:(0771)5883405,E-mail:hy2002-2006@163.com;通信作者:李学坚(1965-),男,博士,教授,高级工程师,硕士生导师,从事中药新产品研究开发。Tel:(0771)3946492.
