摘要
甘草为我国传统常用大宗中药材,分布区域广泛,形态变异幅度大,种质资源的遗传多样性丰富。甘草的种源混乱及基原鉴定困难一直是困扰甘草产业发展的重要因素。本文概述RFLP(restriction fragment length polymorphism,限制性片段长度多态性)、基于PCR的分子标记如RAPD(randomly amplified polymo-rphic DNA,随机扩增多态性DNA)、AFLP(amplification fragment length polymorphism,扩增片段长度多态性)、SSR(simple sequence repeat,简单重复序列)、ISSR(inter-simple sequence repeat,内部简单序列重复)等分子标记技术在甘草基原鉴定和遗传多样性中的研究进展,分析不同分子标记技术的特点及其在甘草中的应用方向,并对分子标记技术在甘草中的应用前景做出展望。
Glycyrrhizae Radix is a traditional commonly used herbal medicine in China,which distributing widely,owning large range of variation in morphology,and rich genetic diversity in germplasm resources.The confusion of seed resource and difficulty of original identification has been always besetting the development of Glycyrrhizae Radix industry.In this review,the research progress of molecular marker technology,such as RFLP,RAPD,AFLP,SSR and ISSR were summarized in term of original identification and genetic diversity.The characteristics of different molecular marker technology and the direction of application in Glycyrrhizae Radix were analyzed,and proposed the expectation for the application prospect of molecular marker technology in Glycyrrhizae Radix identification.
作者
李珍珍
郑司浩
尚兴朴
邓庭伟
王继永
赵润怀
LI Zhen-zhen;ZHENG Si-hao;SHANG Xing-pu;DENG Ting-wei;WANG Ji-yong;ZHAO Run-huai(Jiangxi University of Traditional Chinese Medicine,Nanchang 330004,China;China National Traditional Chinese Medicine Co.,Ltd,Beijing 100195,China)
出处
《中国现代中药》
CAS
2019年第5期684-688,共5页
Modern Chinese Medicine
基金
国家中药标准化项目(ZYBZH-Y-ZY-45)
国家重点研发计划(SQ2017YFC170424)
国家中药材产业技术体系遗传改良研究室建设(CARS-21)
北京市科技计划课题(Z171100001717028)
中医药公益性行业科研专项(201507002-1-10)
关键词
甘草
遗传多样性
分子标记
Glycyrrhizae Radix
genetic diversity
molecular marker
作者简介
通信作者:王继永,研究员,研究方向:濒危药材种质资源保护;Tel:(010)88468089,E-mail:wangjiyong75@163.com;通信作者:赵润怀,研究员,研究方向:中药资源与中药材生产加工;Tel:(010)88468257,E-mail:zhaorunhuai@sina.com.
