摘要
高浓酵母废水的厌氧生物处理效率对废水处理系统的运行成本和经济可行性具有至关重要的作用。该文采用光合细菌强化厌氧污泥的方法处理酵母废水,考察了废水在厌氧处理前后的化学需氧量、色度变化以及光合细菌加入前后微生物的OTU分布、厌氧污泥细菌的物种门类、污泥古菌聚类结果序列数量的变化。结果表明:光合细菌加入后,单段厌氧反应器处理酵母废水化学需氧量和色度去除率分别由原来的58.20%、47.50%显著提升至 75.12%和62.04%,光合细菌强化厌氧生物处理过程效果明显。微生物多样性分析发现在厌氧污泥驯化和添加光合细菌强化过程中,优势菌种不断累积,特异性增强,物种门类数目减少。光合细菌与其他异养细菌存在共生关系,加入光合细菌后污泥中产甲烷优势群落微生物明显增多,从而提高厌氧系统的处理效果。研究结果为高浓工业有机废水的有效处理提供了参考。
Yeast industry provides enormous economic and social benefits for the sugarcane biomass industry. However, yeast industrial wastewater is characterized by high concentration of chemical oxygen demand (CODcr), high chromaticity, high concentration of sulfate and difficult biodegradation, which leads to many issues of the environment. The treatment process of wastewater has the problem that discharge water is difficult to reach the standard and its cost is too high, which has become a bottleneck restricting the development of domestic yeast enterprises. Biotechnology is currently widely used, but the results are still not up to standard. Therefore, biological treatment should be supplemented by other methods for in-depth treatment. In this paper, yeast wastewater was treated by adding photosynthetic bacteria to strengthen anaerobic sludge. The ultrafiltration membrane was used to filter the separated dialysate wastewater as the test object. A small UASB anaerobic reactor was designed. The initial MLSS (mixed liquid sludge concentration) value of the inoculated sludge in the reactor was 4 820 mg/L, and the reactor temperature was kept at 32 ℃. The hydraulic retention time was 48 h, and the influent pH value was controlled between 6.8 and 7.0. At the start-up stage of the anaerobic reactor, glucose, ammonium nitrate and potassium dihydrogen phosphate were used as C, N and P source, respectively, and an appropriate amount of trace elements were added to prepare a nutrient solution with a ratio of 300:5:1 of C:N:P. Yeast wastewater and nutrient solution were mixed and configured into water samples with mass concentration gradient of 3 000-3 500, 7 000-7 500, 10 000-10 500 and 14 000-15 000 mg/L, respectively. The water samples were added to the reactor in a gradient form from low concentration to high concentration, and the batch test was carried out until the organic concentration of wastewater reached the concentration of raw water. After the treatment effect of the reactor was stabilized, the photosynthetic bacteria were added to the domesticated anaerobic sludge, and the concentration of the bacterial suspension was 1.2×10^10 mL^-1 as determined by the Meish turbidimetry method, and the addition amount was 1 mL/L, the inflow was 900-1 000 mL with HRT of 48 h, and the continuous illumination by fluorescent lamp was used. After the reactor was stabilized, the water quality was tested and analyzed after continuous operation for 9 cycles. The CODcr and chromaticity changes of wastewater during anaerobic treatment, the OTU distribution of microorganisms during the addition of photosynthetic bacteria, the species of anaerobic sludge bacteria and sludge, and the changes in the number of archaeological clustering results were investigated. The results showed that the CODCr and chroma removal rates of yeast wastewater treated by anaerobic UASB reactor were significantly increased from 58.20% to 75.12% and 47.50% to 62.04%, respectively, which indicated that the effect of photosynthetic bacteria on the anaerobic biological treatment process has significantly increased. From the analysis of microbial diversity data, it was found that in the process of anaerobic sludge domestication and photosynthetic bacteria optimization, the dominant species was accumulated, the specificity was enhanced, and the number of species was reduced. Photosynthetic bacteria and other heterotrophic bacteria have a symbiotic relationship, and after the addition of the sludge, the dominant microorganisms of methane increased significantly, so as to improve the treatment effect of the anaerobic system. The results of this study provide a technical route for the effective treatment of high-concentration industrial organic wastewater.
作者
李友明
薛宇慧
葛广德
胡永玫
侯轶
Li Youming;Xue Yuhui;Ge Guangde;Hu Yongmei;Hou Yi(State Key Laboratory of Pulp and Paper,South China University of Technology,Guangzhou 510640,China;Guangxi Zhuang Autonomous Region Metallurgical Products Quality Inspection Station,Nanning 530023,China)
出处
《农业工程学报》
EI
CAS
CSCD
北大核心
2019年第9期214-220,共7页
Transactions of the Chinese Society of Agricultural Engineering
基金
广东省科技计划攻关项目(2015A020215009)
关键词
废水
废水处理
光合细菌
厌氧处理工艺
微生物多样性
wastewater
wastewater disposal
photosynthetic bacteria
anaerobic treatment process
microbial diversity
作者简介
李友明,博士,教授,博士生导师,主要从事食品工业清洁生产技术的研究。Email:ymli3@scut.edu.cn;通信作者:侯轶,博士,教授级高工,主要从事食品行业高浓度有机废水高值化利用和处理研究。Email:ceyhou@scut.edu.cn.
