摘要
背景:细胞黏附性质影响细胞的迁移、增殖、分化,对于细胞分离和富集、组织工程、临床疾病研究及应用均有极其重要的意义。微流控芯片具有小型化、集成化、高通量、低能耗、分析快速等特性,在细胞黏附研究中具有独特的优势。目的:总结并讨论微流控芯片技术在细胞黏附研究中应用的最新进展。方法:由第一作者检索2008至2018年PubMed数据库和百链云数据库,纳入与微流控技术、细胞黏附等相关的文献,并进行系统整理、归纳总结和分析。结果与结论:共检索到文献238篇,按照纳入和排除标准筛选后,共纳入53篇文献,通过阅读、总结和分析文献发现:微流控芯片平台为进行体外基于细胞黏附研究提供了新的可能性,其具有更高的通量,并且能精确控制生物、物理、化学因素构建体内细胞微环境,调控一系列细胞行为,包括细胞黏附、迁移、生长、增殖和分化及细胞与细胞、细胞与基质的相互作用等。微流控芯片技术在动态监测细胞黏附过程、定量检测细胞黏附力、分离和富集稀有细胞、筛选生物医学材料及研究细胞黏附相关疾病等方面具有广阔的应用前景。
BACKGROUND:Cell adhesion affects cell migration,proliferation,and differentiation.It is of extremely vital significance for cell separation and enrichment,tissue engineering and clinical disease research.Microfluidic chips have unique advantages in cell adhesion studies such as miniaturization,integration,high throughput,low energy consumption,and rapid analysis.OBJECTIVE:To summarize and discuss the latest advances of microfluidic chip technology in cell adhesion research.METHODS:The first author performed a data retrieval of PubMed and Bailianyun databases to search the articles published during 2008-2018 and addressing cell adhesion and microfluidic chip technology and reviewed the literatures systematically.RESULTS AND CONCLUSION:A total of 238 articles were retrieved and 53 articles were included in the final analysis according to the inclusion and exclusion criteria.These articles were read,summarized and analyzed.Results showed that the microfluidic chip platform provides new possibilities for in vitro studies of cell adhesion because it exhibits higher throughput,enables precise control of the biological,physical,and chemical factors involved in the construction of in vivo cellular microenvironment,and regulates a series of cellular behaviors including cell adhesion,migration,growth,proliferation,differentiation,cell-cell interactions,and cell-matrix interactions.Microfluidic chip technology has broad application prospects in the dynamic monitoring of cell adhesion process,quantitative measurement of cell adhesion,isolation and enrichment of rare cells,screening of biomedical materials,and research on cell adhesion-related diseases.
作者
专行
郑国侠
王云华
Zhuan Hang;Zheng Guoxia;Wang Yunhua(School of Medicine,Dalian University,Dalian 116622,Liaoning Province,China;School of Environmental and Chemical Engineering,Dalian University,Dalian 116622,Liaoning Province,China)
出处
《中国组织工程研究》
CAS
北大核心
2019年第22期3584-3590,共7页
Chinese Journal of Tissue Engineering Research
基金
国家自然科学基金(81471807),项目负责人:王云华
国家自然科学基金(41476085),项目负责人:郑国侠~~
关键词
细胞黏附
微流控芯片技术
细胞黏附力
定量检测
细胞分离和富集
组织工程
生物医学材料
体外模型重建
药物筛选
国家自然科学基金
cell adhesion
microfluidic chip technology
quantitative measurement
cell isolation and enrichment
tissue engineering
biomedical materials
in vitro model reconstruction
drug screening
the National Natural Science Foundation of China
作者简介
专行,女,1993年生,湖北省咸宁市人,汉族,大连大学在读硕士,主要从事微流控芯片在病原生物学中的应用研究;通讯作者:王云华,副教授,硕士生导师,大连大学医学院,辽宁省大连市116622.
