摘要
细胞的三维培养是目前的研究热点,其主要依赖于微尺寸下水凝胶细胞支架的制备。常规的3D打印是常用的细胞支架制备技术,但其无法同时满足打印范围和打印精度的需求,且存在打印速度慢的局限性,这限制了高精度、较大范围细胞3D打印技术的发展。基于数字光处理(DLP)技术,搭建了一个成本低、打印范围较大、精度在微米级的细胞打印平台。该平台包括数字动态掩膜投影光学系统、实时检焦光学系统和高精度运动系统三个部分。通过聚乙二醇双丙烯酸酯(PEGDA)水凝胶微结构的紫外固化实验,证明该平台实际打印精度可达25μm。该平台可通过精确控制高精度位移平台的移动,实现较大范围打印。为验证细胞打印的可行性,使用搭建的平台完成了甲基丙烯酸化水凝胶(GelMA)生物细胞支架的打印,并完成了人肝细胞系HL7702细胞的三维培养。该平台未来可用于组织和器官打印与高通量药物筛选领域。
The three-dimensional cell culture is one of the research focuses currently,and mainly depends on the fabrication of hydrogel cell scaffolds in micro-scale.The conventional 3 D printing is commonly preparation technology of cell scaffolds,but can not simultaneously meet the needs of printing range and printing accuracy and has a limitation of slow printing speed,thus limiting the development of 3 D cell printing technology with high precision and wide range.Based on the digital light processing(DLP)technology,a micro-level cell printing platform with low cost and large printing range was built.The platform consists of the digital dynamic mask projection optical system,real-time focus detection optical system and high precision motion system.The ultraviolet curing experiment of the polyethylene glycol diacrylate(PEGDA)hydrogel microstructure proves that the actual printing accuracy of the platform reaches 25μm.The platform can realize printing with a wide print range by accurately controlling the movement of the high precision displacement platform.To verify the feasibility of cell printing,a methacrylate hydrogel(GelMA)biological cell scaffold was printed by using the platform,and the 3 D culture of human liver cell line HL7702 cells was completed.The platform can be used for tissue and organ printing and high-throughput drug screening in the future.
作者
何希文
王疏影
张楚
韩超
陈翔
He Xiwen;Wang Shuying;Zhang Chu;Han Chao;Chen Xiang(Department of Micro/Nano Electronics,Shanghai Jiao Tong University,Shanghai 200240,China)
出处
《微纳电子技术》
北大核心
2020年第3期216-222,共7页
Micronanoelectronic Technology
基金
上海市科委基础重大研发计划资助项目(17JC1401001).
作者简介
何希文(1995—),男,湖南郴州人,硕士研究生,主要研究方向为微光机电系统与微流控芯片;通信作者:陈翔(1974—),男,湖北黄冈人,博士,副研究员,硕士研究生导师,主要研究方向为塑性材料微流控芯片加工与封装技术、纳米材料及微流体技术在快速疾病诊断中的应用、微流体在细胞操控分离中的应用等。E-mail:xiangchen@sjtu.edu.cn
