摘要
目的探讨E盒结合锌指蛋白2(ZEB2)在卵巢癌细胞侵袭及迁移中的作用及机制。方法将卵巢癌细胞SKOV3分为Control组、ZEB2 shRNA组、shRNA-NC组。ZEB2 shRNA组、shRNA-NC组分别转染ZEB2 shRNA和shRNA-NC,Control组不进行细胞转染。采用实时聚合酶链反应(RT-PCR)和蛋白质印迹(Western blot)法检测转染效果,细胞划痕实验检测细胞迁移能力,Transwell小室检测细胞侵袭能力,Western blot法检测迁移和侵袭相关蛋白基质金属蛋白酶2(MMP2)、波形蛋白(vimentin)的相对表达量。结果 ZEB2 shRNA组细胞ZEB2mRNA和蛋白相对表达量均低于shRNA-NC组和Control组(P﹤0.05)。ZEB2 shRNA组细胞迁移率均低于Control组和shRNA-NC组,侵袭细胞数目均少于Control组和shRNA-NC组,差异均有统计学意义(P﹤0.05)。ZEB2 shRNA组细胞中MMP2、vimentin蛋白相对表达量均低于Control组和shRNA-NC组,差异均有统计学意义(P﹤0.05)。结论沉默ZEB2基因表达能够抑制卵巢癌细胞迁移和侵袭能力,MMP2和vimentin可能是其作用机制之一。
Objective To investigate the effect and mechanism of zinc finger E-box binding homeobox 2 (ZEB2) in invasion and migration of ovarian cancer cells.Method Ovarian cancer cells SKOV3 were divided into Control group, ZEB2 shRNA group and shRNA-NC group.Cells in ZEB2 shRNA group and shRNA-NC group were respectively transfected with ZEB2 shRNA and shRNA-NC,while those in Control group were not transfected.Real-time polymerase chain reaction (RT-PCR) and Western blot were used to detect transfection effects,cell scratch test was adopted for cell migration ability,Transwell chamber for cell invasion ability,and Western blot for relative expression of matrix metalloproteinase 2 (MMP2) and vimentin associated with migration and invasion.Result Relative expression of ZEB2 mRNA,ZEB2, MMP2 and vimentinin protein,cell migration rate,number of invading cells in ZEB2 shRNA group were all lower than those in shRNA-NC group and Control group,and the differences were statistically significant (P<0.05).Conclusion Silencing of ZEB2 gene expression inhibits the migration and invasion of ovarian cancer cells,and MMP2 and vimentin may be involved in part of its action mechanism.
作者
张宁
左丽
肖谜
应江山
ZHANG Ning;ZUO Li;XIAO Mi;YING Jiangshan(Department of Medical Oncology,Minhang Branch of Cancer Hospital Affiliated to Fudan University (Tumor Hospital of Minhang District,Shanghai),Shanghai 200240,China)
出处
《癌症进展》
2019年第7期780-783,共4页
Oncology Progress
关键词
卵巢癌
迁移
侵袭
E盒结合锌指蛋白2
ovarian cancer
migration
invasion
zinc finger E-box binding homeobox 2
作者简介
通信作者(corresponding author):应江山,邮箱:cinder_zhang@163.com.
