摘要
植物根际促生菌(Plant growth-promoting rhizobacteria,PGPR)具有抵抗植物病害,促进植物生长等作用。以前期在山东省金乡县连作大蒜根际土壤中分离获得的根际细菌为研究对象,筛选获得对大蒜根腐病病原菌H5(Setophoma terrestris)和H9 (Fusarium solani)具有较好拮抗效果的菌株DS7,经16S rRNA基因序列分析初步鉴定,该菌株属于芽孢杆菌属(Bacillus)。功能特性研究显示:菌株Bacillus sp. DS7对H5抑制率达到51.72%,对H9抑制率达到42.31%;菌株DS7具有产IAA(19.29±0)μg/mL、产铁载体、水解蛋白、溶磷等能力,但不具有产ACC脱氨酶能力。田间接种试验结果显示:与不接菌的对照相比,接种菌株DS7后大蒜产量提高15.42%;土壤的蔗糖酶活性和脲酶活性高于对照土壤,过氧化氢酶活性低于对照土壤。综上,植物根际促生菌Bacillus sp. DS7具有多种功能特性,可以增加田间大蒜产量,改良土壤,是潜在的微生物肥料生产菌种。
Plant growth-promoting rhizobacteria(PGPR)has the function of resisting plant diseases and promoting plant growth.The rhizosphere bacteria previously isolated from the rhizosphere soil of garlic in continuous cropland in Jinxiang County of Shandong Province were studied.The results showed that the selected strains had good antagonism on the pathogens H5(Setophoma terrestris)and H9(Fusarium solani)of garlic root rot.The strain DS7 was initially identified by the sequence analysis of the 16S rRNA gene,which belongs to the genus Bacillus.The study of functional properties showed that the inhibition rate of strain Bacillus sp.DS7 inhibition to H5 reached 51.72%,and the inhibition rate to H9 reached 42.31%.The strain DS7 carrys the abilities of producinged IAA(19.29±0)μg/mL,producing iron-producing carrier,hydrolyzing ed protein,and dissolving phosphorus-dissolving ability,but does can’t have the ability to produce ACC deaminase.The field inoculation test results showed that the garlic yield increased by 15.42%after inoculation with strain DS7,and the invertase activity and urease activity of soil were higher than that of the control soil,and the catalase activity was lower than that of the control soil.In summary,the plant rhizosphere growth-promoting strain Bacillus sp.DS7 has a variety of functional properties,which can increase the yield of garlic in the field and improve the soil.It is a potential microbe fertilizer production strain.
作者
崔曼
尹彦舒
张梦琦
卜宁
陈云云
高淼
马莲菊
CUI Man;YIN Yan-shu;ZHANG Meng-qi;BU Ning;CHEN Yun-yun;GAO Miao;MA Lian-ju(College of Life Science,Shenyang Normal University,Shenyang Liaoning 110034;Institute of Agricultural Resources and Regional Planning,Chinese Academy of Agricultural Sciences/Key Laboratory of Microbial Resources Collection and Preservation,Ministry of Agriculture and Rural Affairs,Beijing 100081)
出处
《中国土壤与肥料》
CAS
CSCD
北大核心
2019年第1期173-179,共7页
Soil and Fertilizer Sciences in China
关键词
大蒜
根际细菌
功能特性
土壤酶活
garlic
plant growth-promoting rhizobacteria
functional characterizations
soil enzyme activies
作者简介
崔曼(1994-),女,辽宁辽阳人,硕士研究生,主要从事微生物资源与应用研究。E-mail:1224772821@qq.com;通讯作者:高淼,E-mail:gaomiao@caas.cn;通讯作者:马莲菊,E-mail:744289467@qq.com.
