摘要
以秘鲁野生细叶番茄为试材,采用不同植物激素组合及不同光照对比培养方法,对野生番茄愈伤组织诱导及愈伤生长特性进行了研究。结果表明,MS+6-BA (0.05～2.0) mg/L+NAA 0.5 mg/L、MS+NAA 0.5 mg/L或2,4-D 0.5 mg/L均可诱导叶片及茎段愈伤化。不同光照条件(强光、弱光及黑暗培养)下愈伤发生颜色变化。无论光照或暗培养,愈伤组织增生速度均较快,20 d生长量为原来的3～14倍。根据定性和定量调查确定MS+NAA 0.5 mg/L为秘鲁野生细叶番茄愈伤光培养或暗培养的适宜培养基。该研究为野生番茄在基因工程上的利用奠定了技术基础。
Taking a wild tomato variety Solanum pimpinellifolium as experiment material,the effect of different phytohormone combination and different light intensity on callus growth were studied.The results showed that the medium MS+6-BA(0.05-2.0)mg/L+NAA0.5mg/L,MS+NAA0.5mg/L(or2,4-D0.5mg/L)were all suitable for leaf and shoot callus induction.The callus changed it’s color under different light(strong light,low light and dark).No matter what kind of light,the callus can grow very fast with the speed of3-14times of the original weight.According to qualitative and quantitive investigation,MS+NAA0.5mg/L was assured as the suitable medium for S.pimpinellifolium callus culture under light or dark condition.This research laid a technological foundation for the genetic engineering of wild tomato.
作者
石颖
王桂英
王长娜
刘侠
阚玉文
郜秋华
张恭
车文利
庞国新
SHI Ying;WANG Gui-ying;WANG Chang-na(Langfang Academy of Agriculture and Forestry Sciences,Langfang,Hebei065000;Langfang Vocational and Technical College,Department of Urban Construction Engineering,Langfang,Hebei065000)
出处
《安徽农业科学》
CAS
2018年第35期47-49,共3页
Journal of Anhui Agricultural Sciences
作者简介
石颖(1968-),女,河北廊坊人,高级农艺师,从事作物育种及植物组织培养工作。
