摘要
目的观察电针少阳经特定穴对于硝酸甘油(nitroglycerin,NTG)诱导的偏头痛(migraine,ME)大鼠模型血清中一氧化氮(NO)和内皮素(endothelin-1,ET-1)含量及50%缩足阈值(paw withdrawal threshold,PWT)的影响,比较电针少阳经特定穴和非经非穴在治疗ME大鼠的疗效差异。方法健康SD大鼠40只,随机分为空白组(A组)、模型组(B组)、电针少阳经特定穴治疗组(C组)和电针非经非穴治疗组(D组),每组10只。B、C、D组按10 mg/kg臀部皮下注射NTG造模,A组臀部皮下注射等量生理盐水。造模后30 min,C组和D组分别行相应电针治疗30 min,测量各组大鼠不同阶段的50%PWT,采用硝酸还原酶法和ELISA分别检测大鼠血清中NO和ET-1含量。结果造模后30 min:与造模前30 min比较,B、C、D组50%PWT显著降低(P<0.01)。治疗后30 min:与造模后30 min比较,C、D组的50%PWT显著升高(P<0.01);与D组比较,C组50%PWT显著升高(P<0.01)。与A组比较,B组大鼠血清中的NO、ET-1的含量显著增高(P<0.01);与B组比较:C组大鼠血清中的NO、ET-1含量显著降低(P<0.01),而D组大鼠血清中的NO、ET-1含量差异无统计学意义(P>0.05);与D组比较,C组大鼠血清中的NO、ET-1含量显著降低(P<0.01)。结论电针少阳经特定穴在治疗NTG诱导的ME大鼠模型中,能显著降低大鼠血清中NO、ET-1的含量,提高大鼠50%PWT,疗效明显优于电针非经非穴,该结果为临床应用电针少阳经特定穴治疗偏头痛提供一定的实验依据。
Objective To observe the effect of electroacupuncture at specific acupoints of Shaoyang meridians on serum nitric oxide(NO)and endothelin-1(ET-1)levels and50%paw withdrawal threshold(PWT)in a rat model of nitroglycerin(NTG)-induced migraine,and to compare the therapeutic efficacy of electroacupuncture at specific acupoints of Shaoyang meridians and non-meridian/acupoint in the treatment of rats with migraine.Methods A total of40healthy Sprague-Dawley rats were equally and randomly divided into four groups:blank group(group A),model group(group B),group C,which was treated with electroacupuncture at specific acupoints of Shaoyang meridians,and group D,which was treated with electroacupuncture at non-meridian/acupoint.The rats in groups B,C,and D were injected subcutaneously with NTG at the hip at a dose of10mg/kg for modeling,and those in group A were injected subcutaneously with the same amount of normal saline.At30min after modeling,groups C and D were treated with corresponding electroacupuncture for30min.The50%PWT of rats at different stages in each group was measured.Serum levels of NO and ET-1were measured by the nitrate reductase method and ELISA.Results At30min after modeling,groups B,C,and D had a significant reduction in50%PWT(P<0.01).At30min after treatment,groups C and D had a significant increase in50%PWT(P<0.01);compared with group D,group C had significantly increased50%PWT(P<0.01).Compared with group A,group B showed significantly increased serum levels of NO and ET-1(P<0.05).Compared with group B,group C exhibited significantly reduced serum levels of NO and ET-1(P<0.05),but there were no significant differences in serum levels of NO and ET-1between group B and group D(P>0.05).Compared with group D,group C showed significant reductions in serum levels of NO and ET-1(P<0.01).Conclusion In the treatment of NTG-induced migraine model in rats,electroacupuncture at specific acupoints of Shaoyang meridians can significantly reduce the levels of NO and ET-1in serum and increase50%PWT,it has a significantly better therapeutic effect than electroacupuncture at non-meridian/acupoint,and it provides an experimental basis for the clinical application of electroacupuncture at specific points of Shaoyang meridians to treat migraine.
作者
叶意红
刘秋佳
康乐蔷薇
王乾娜
刘未艾
岳增辉
贺新民
陈建宏
YE Yihong;LIU Qiujia;KANG Leqiangwei;WANG Qianna;LIU Weiai;YUE Zenghui;HE Xinmin;CHEN Jianhong(Hunan University of Chinese Medicine, Changsha, Hunan 410021, China;Hengyang Hospital of Traditional Chinese Medicine, Hengyang, Hunan 421001, China)
出处
《湖南中医药大学学报》
CAS
2018年第12期1453-1457,共5页
Journal of Hunan University of Chinese Medicine
基金
中国博士后面上项目基金(2017M612566)
湖南省中医药科研计划项目(2017125)
湖南省研究生科研创新项目(CX2017B437)
关键词
偏头痛
硝酸甘油
电针
少阳经特定穴
非经非穴
一氧化氮
内皮素
migraine
nitroglycerin
electroacupuncture
specific acupoints of Shaoyang meridians
non-meridian/acupoint
nitric oxide
endothelin-1
作者简介
叶意红,男,在读硕士研究生,研究方向:针灸治病机制;通讯作者:刘未艾,女,博士,博士后,主任医师,E-mail:55999630@qq.com;通讯作者:岳增辉,男,教授,博士研究生导师,E-mail:624755064@qq.com。
