摘要
该实验意在探究痰热清注射液入脑成分在脑部疾病中的应用。采用液质联用技术对痰热清注射液成分及其在大鼠脑脊液中存在的成分进行定性分析,运用生物信息学相关理论知识预测痰热清入脑成分对脑部疾病可能的作用机制。共发现了痰热清17个入脑成分,筛选到222个核心靶点,生物过程包括凋亡过程的负调节、MAPK级联、Ras蛋白信号转导等31个条目;信号通路包括PI3K-Akt信号通路、MAPK信号通路、神经营养因子信号通路等22个条目。通过预测痰热清入脑成分作用的脑部疾病,富集结果筛选出包括脑中风、偏头痛、脑膜瘤等9种脑部疾病,为今后深入研究某一脑病提供思路和方向,为进一步揭示其分子机制奠定理论基础。
The study explores the application of Tanreqing Injection into brain components in brain diseases.The components of Tanreqing Injection and its existing components in rat cerebrospinal fluid were qualitatively analyzed by liquid chromatography-mass spectrometry(LC-MS).The possible mechanism of action of Tanreqing Injection into brain on brain diseases was predicted by network pharmacological theory.In this study,17 brain-entry components of Tanreqing Injection were founded,and 222 core targets were obtained from network pharmacological results.The biological processes include 31 items such as negative regulation of apoptotic process,MAPK cascade,Ras protein signal transduction,and 22 items such as PI3 K-Akt signal transduction,MAPK signal transduction and neurotrophic factor signal transduction.Nine brain diseases including stroke,migraine and meningioma were screened out by predicting the effect of Tanreqing Injection on brain components,which provide ideas and directions for further study of a certain encephalopathy and lay a theoretical foundation for further revealing its molecular mechanism.
作者
吕忠民
王毅
王丹巧
李涛
张海鸣
聂颖兰
宋福江
孙健
LYU Zhong-min;WANG Yi;WANG Dan-qiao;LI Tao;ZHANG Hai-ming;NIE Ying-lan;SONG Fu-jiang;SUN Jian(Department of Pharmacology,Basic Medical College,Heilongjiang University of Traditional Chinese Medicine,Harbin 150040,China;State Key Laboratory Breeding Base of Dao-di Herbs,Experimental Research Center,China Academy of Chinese Medical Sciences,Beijing 100700,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2020年第4期937-945,共9页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(81102672)
中央级公益性科研院所基本科研业务费专项(ZZ2016013,GH2018001).
关键词
痰热清注射液
药物入脑成分
生物信息学
Tanreqing Injection
pharmaceutical ingredients in brain composition
bioinformatics
作者简介
通讯作者:孙健,研究员,研究方向为中药物质基础及活性机制,E-mail:sunjiankw@163.com;通讯作者:宋福江,硕士研究生,研究方向为药物新剂型、新技术、新药研发,E-mail:sfbpdm@163.com;吕忠民,硕士研究生,E-mail:1040999402@qq.com
