摘要
目的运用气相色谱-质谱联用技术对党参Codonopsis pilosula (Franch.) Nannf、素花党参Codonopsis pilosula Nannf.var. modesta (Nannf.) L. T. Shen及川党参Codonopsis tangshen Oliv.的游离糖成分进行分析鉴定。方法采用震荡提取法从党参中提取多种游离糖化合物,游离糖成分经乙酰化后利用气相色谱-质谱法分析其组成、结构。结果通过气相色谱-质谱分析结合标准品数据以及相关文献,鉴别出3种党参中7种游离糖成分,分别为山梨糖、半乳糖、葡萄糖、D-甘露糖、果糖、肌醇、蔗糖,游离糖含量存在差异。结论气相色谱-质谱联用技术可以对党参游离糖成分进行定性及定量分析,为党参品质分析提供科学依据。
Objective To identify the free sugar components in three species of Codonopsis[Codonopsis pilosula(Franch.)Nannf.,Codonopsis pilosula Nannf.var.modesta(Nannf.)L.T.Shen,and Codonopsis tangshen Oliv.]by gas chromatography-mass spectrometry(GC-MS).Methods A variety of free sugar compounds were extracted from Codonopsis by shaking extraction.The composition and structure of free sugar components were analyzed by GC-MS after acetylation.Results Seven free sugar components were identified in the three species of Codonopsis by GC-MS according to the data of reference standard and the relevant literature,including sorbose,galactose,glucose,D-mannose,fructose,inositol,and sucrose.There were differences in the content of these free sugar components between the three species of Codonopsis.Conclusion GC-MS can be used for the qualitative and quantitative analysis of free sugar components in Codonopsis.It can provide a scientific basis for the quality analysis of Codonopsis.
作者
李家宇
左之文
王曦璠
陈林
王玺
贺炜
周卿意骏
张水寒
LI Jiayu;ZUO Zhiwen;WANG Xifan;CHEN Lin;WANG Xi;HE Wei;ZHOU Qingyijun;ZHANG Shuihan(Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Hunan Academy of Chinese Medicine, Changsha, Hunan 410013, China;Dingxi Codonopsis Dryness and Processing Engineering Technology Research Center, Dingxi, Gansu 748200,China;Naval Air University, Yantai, Shandong 264000, China;Resgreen Group, Changsha, Hunan 410329, China)
出处
《湖南中医药大学学报》
CAS
2018年第12期1398-1402,共5页
Journal of Hunan University of Chinese Medicine
基金
特色中药资源综合开发和利用研究(2060302-1607-01)
关键词
党参
乙酰化
游离糖
气相色谱-质谱联用
Codonopsis
acetylation
free sugar
gas chromatography-mass spectrometry
作者简介
李家宇,男,在读硕士研究生,研究方向:中药资源质量标准及制剂研究;通讯作者:张水寒,女,研究员,博士研究生导师,E-mail:592249880@qq.com。
