摘要
为了筛选磺化钻井液高效降解菌株,以新疆某磺化钻井液伤害土壤为菌源,磺化钻井液为唯一碳源,通过富集驯化分离筛选出一株降解磺化钻井液效率较高的菌株Xh8,经鉴定为Bacillus flexus。经生理生化及16S rRNA基因序列分析确定了其分类地位,并进行了单因素和正交试验以确定其降解特性。单因素试验表明Xh8生长最佳条件为:pH值为9.5、盐质量分数为4%、转速为150 r/min、温度为35℃、接种量为10%、N源蛋白胨、P源K2HPO4。正交试验表明磺化钻井液COD降解最佳条件为:pH值为9.5、盐质量分数为4.5%、转速为150 r/min、温度为35℃。培养7 d,磺化钻井液COD降解率为22.9%。试验结果表明,Xh8可作为磺化钻井液降解的优良备选菌株。该研究可为进一步治理受到磺化钻井液伤害的地层提供理论指导。
In order to screen out the bacteria strain that can degrade sulphonated drilling fluid efficiently,a series of studies were carried out with the sulphonated drilling fluid as the only carbon source and some soil contaminated by the sulphonated drilling fluid in Xinjiang as the bacteria source.The bacteria strain Xh8that can degrade sulphonated drilling fluid more efficiently was screened out by means of enrichment,acclimation and separation.And according to the evaluation,it is Bacillus flexus.Then,its classification status was determined by virtue of physiological&biochemical and16S rRNA gene sequence analysis.In addition,its degradation property was figured out through single-factor experiment and orthogonal experiment.The single-factor experiment results show that the growth of Xh8is optimal in the condition of pH value9.5,salt mass fraction4%,rotation speed150r/min,temperature35℃,inoculation amount10%,N-source peptone,and P-source K2HPO4.The orthogonal experiment results show that the optimal COD degradation condition of sulphonated drilling fluid is pH value9.5,salt mass fraction4.5%,rotation speed150r/min and temperature35℃.After being cultured for7days,the COD degradation rate of sulphonated drilling fluid is22.9%.The experimental results indicate that Xh8can be taken as the preferred bacteria strain for the degradation of sulphonated drilling fluid.The research results can provide the theoretical guidance for the further treatment of the strata contaminated by sulphonated drilling fluid.
作者
宋淑芬
马立安
胡传炯
张照明
周宏
余维初
SONG Shufen;MA Li’an;HU Chuanjiong;ZHANG Zhaoming;ZHOU Hong;YU Weichu(State Key Laboratory of Petroleum Pollution Control, Beijing 102206, Chin;Yangtze University, Jingzhou 434023, Hubei, China;Hubei Collaborative Innovation Center for Unconventional Oil and Gas, Wuhan 434000,Hubei, China;Dongying City Kewei Biotechnology L.td, Dongying 257091, Shandong, China;Beijing Design Branch of China Petroleum Engineering Construction Co., Ltd, Beijing 100101, China)
出处
《石油钻采工艺》
CAS
CSCD
北大核心
2018年第5期589-595,共7页
Oil Drilling & Production Technology
基金
石油石化污染物控制与处理国家重点实验室开放课题"聚磺钻井液废液及钻屑土壤化作用机理研究"(编号:PPC2016004)
关键词
磺化钻井液
降解菌
筛选
降解特性
弯曲芽孢杆菌
钻井废液
无害化处理
sulphonated drilling fluid
Degrading bacteria
screening
degradation characteristic
Bacillus flexus
waste drilling fluid
harmless treatment
作者简介
第一作者:宋淑芬(1993-),2016 年毕业于长江大学生物工程专业,在读硕士研究生,研究方向为环境微生物学。通讯地址:(434025)湖北省荆州市荆州区荆秘路88 号。电话:15826644594。E-mail:760100785@qq.com;通讯作者:余维初(1965-),二级教授,博士生导师,从事油气田应用化学方向的研究与教学工作。E-mail:yuweichu@126.com。
