摘要
为获得更加适应自然环境的乙草胺降解菌株,以MSM基础盐培养基从常年经受乙草胺污染的农田土壤中分离乙草胺降解菌,筛选高效降解菌株,并对其进行鉴定及生长和降解特性分析。结果表明,共分离得到乙草胺降解细菌12株,通过高效液相色谱法定量检测确定菌株ACD-9的降解效率最高,3 d内对初始浓度为50·mg·L-1的乙草胺降解率可达到56.78%;从菌落特征、生理生化特性以及16S r RNA基因序列3个方面分析,鉴定菌株ACD-9为芽孢杆菌(Bacillus sp.);菌株ACD-9可在温度4～50℃、pH值4.5～10.0、NaCl质量浓度为0～150 g·L-1的条件下生长,其最适条件为46℃,pH值5.0～6.0,NaCl 0～10 g·L-1。综合而言,菌株ACD-9环境耐受力较好,适应能力较强,具有良好的应用前景。
To obtain bacterial strains that could adapt a wide range of natural environment conditions, mineral salts medium （MSM） was used to isolate acetochlor-degrading bacteria from the soils that were subject to acetochlor contamination many years. The strain with higher acetochlor-degrading efficiency was screened and identified, and the growth and acetochlor degrading characteristics were also studied. The resuhs showed that 12 acetochlor-degrading bacterial strains were obtained in total, in which ACD-9 was certified as the highest efficiency strain through high performance liquid chromatography （HPLC） method, and the 3^nd day acetochlor degrada-tion rate was 56.78% for 50 mg· L^-1 acetochlor of in the liquid. According to the resuhs of colony morphology, physiological and bio-chemical characteristics, and 16S rRNA gene sequence analyses, ACD-9 was identified as a member of Bacillus. ACD-9 could grow under the condition of 4~50 ℃, pH 4.5~10.0, and 0~150 g·L^-1 NaC1, while the optimal condition was 46 ℃, pH 5.0~6.0,0~10 μg·L^-1 NaC1. In conclusion, ACD-9 had good tolerance and adaptation for environment, which could be applied with a good prospect.
作者
王苑力
李海峰
符加珂
张月阳
屈建航
田海龙
WANG Yuanli;LI Haifeng;FU Jiake;ZHANG Yueyang;QU Jianhang;TIAN Hailong(College of Biological Engineering,Henan University of Technology,Zhengzhou,Henan 450001,China)
出处
《天津农业科学》
CAS
2018年第12期78-81,共4页
Tianjin Agricultural Sciences
基金
国家自然科学基金项目(31400103
31370147)
河南省高校科技创新团队支持计划项目(151RTSTHN019)
关键词
乙草胺
降解菌
芽孢杆菌
耐盐性
acetochlor
degrading bacteria
Bacillus
salt tolerance
作者简介
王苑力（1994-），男，山西原平人，在读硕士生，主要从事食品加工与安全研究。;通讯作者：李海峰（1984-），女，河北涿鹿人，副教授，硕士生导师，主要从事微生物与磷循环研究。
