摘要
建立高效液相色谱-蒸发光散射检测器法(high performance liquid chromatography-evaporative light scattering detector,HPLC-ELSD)同时测定保健食品银杏叶刺梨胶囊中银杏萜类内酯含量的方法。色谱柱为Waters Symmetry C18色谱柱(250 mm×4.6 mm,5μm),柱温为35℃;流动相为甲醇-水=30∶70(体积比),流速1.0 mL/min;ELSD检测器雾化温度100℃,雾化气为空气,气流速度2.5 L/min。白果内酯、银杏内酯A、银杏内酯B、银杏内酯C分别在4.376μg～3.128μg,4.176μg～12.528μg,4.320μg～12.960μg,4.368μg～13.104μg之间线性关系良好,平均加样回收率分别为100.86%、101.52%、99.60%、100.69%。该方法可准确测定银杏叶刺梨胶囊中银杏萜类内酯的含量,可用于该产品的质量分析。
A HPLC-ELSD method was developed to determine the content of Ginkgo Terpene Lactones in health food ginkgo leaf and Rosa roxburghii capsule.The HPLC was performed on Waters Symmetry C18(250 mm×4.6 mm,5μm)column,mobile phase consisted methanol-H2O(30∶70,volume ratio),column temperature was set at 35℃,and flow rate was 1.0 mL/min.The temperature of drift tube was 100℃and the flow rate of gas was 2.5 L/min.A good linearity was obtained in the range of 4.376μg-13.128μg of bilobalide,4.176μg-12.528μg of ginkgolide A,4.320μg-12.960μg of ginkgolide B,4.368μg-13.104μg of ginkgolide C.The average recovery rates of bilobalide,ginkgolide A,ginkgolide B,and ginkgolide C were 100.86%,101.52%,99.60%,and 100.69%,respectively.The established method was suitable for quality control of ginkgo leaf and Rosa roxburghii capsule.
作者
周亚杰
冯鹏
郭原
冯敏
ZHOU Ya-jie;FENG Peng;GUO Yuan;FENG Min(Nanjing Zhongke Pharmaceutical Co.,Ltd.,Nanjing 210008,Jiangsu,China)
出处
《食品研究与开发》
CAS
北大核心
2018年第23期158-163,共6页
Food Research and Development
关键词
高效液相色谱法
银杏叶刺梨胶囊
萜类内酯
含量测定
质量分析
high performance liquid chromatography
ginkgo leaf and Rosa roxburghii capsule
ginkgolides
determination of content
quality analysis
作者简介
周亚杰(1984—),男(汉),高级工程师,本科,研究方向:天然活性产物的分离和分析;通信作者:冯鹏,硕士生导师,教授,主要从事灵芝孢子的功能研究。
