摘要
【目的】研究解磷菌PSBHY-3对池塘底泥的解磷效果,为解磷菌在养殖池塘中的推广应用提供参考依据。【方法】以模拟池塘环境系统为平台,探究不同浓度(103、104、105和106CFU/mL)PSBHY-3对池塘底泥的解磷效果;并在无菌池塘底泥水体系统中分别设P组(PSBHY-3)、D组(有机物降解菌De)、P-D组(PSBHY-3与De的初始菌量比1∶1)、P-3D组(PSBHY-3与De的初始菌量比1∶3)、3P-D组(PSBHY-3与De的初始菌量比3∶1)和对照组,分析PSBHY-3与De复配使用的解磷效果。【结果】至试验结束(第8 d)时,与对照组相比,J-3(103CFU/mL)、J-4(104CFU/mL)、J-5(105CFU/mL)和J-6(106CFU/mL)组的池塘底泥总磷(TP)含量均显著降低(P<0.05,下同),对应的TP降解率分别为7.05%、6.60%、4.03%和9.17%。在PSBHY-3和De复配试验中,2株菌株间不仅未出现生长拮抗现象,还能促进De生长。至试验结束(第8 d)时,各加菌组对池塘底泥TP的降解率均显著高于对照组,其中,以P组的TP降解率(8.17%)最高,而P-3D、P-D、D和3P-D组的TP降解率分别为5.38%、4.97%、4.38%和3.91%,各加菌组间均无显著差异(P>0.05)。经16S rDNA序列分析和Biolog微生物自动分析系统鉴定,确定PSBHY-3为解淀粉芽孢杆菌(Bacillus amyloliquefaciens)。【结论】PSBHY-3为解淀粉芽孢杆菌,对池塘底泥沉积磷具有较好的降解效果,可作为研发水产养殖用解磷菌菌剂的备选菌株。
【Objective】Effects of phosphate solubilizing bacteria PSBHY-3 on pond sediment were studied to provide reference for the extension and application of phosphate solubilizing bacteria in ponds.【Method】Based on the platform of simulated pond environment system,phosphorus removal effects of PSBHY-3 in different concentrations(10^3,10^4,10^5 and 10^6 CFU/mL)were explored.In the sterile muddy-water system of pond,group P(PSBHY-3),group D(organic degradation bacteria De),group P-D(PSBHY-3 and De with an initial bacterial ratio of 1∶1),group P-3D(PSBHY-3 and De with an initial bacterial ratio of 1∶3),group 3P-D(PSBHY-3 and De with an initial bacterial ratio of 3∶1)and control group were set up,and the phosphate solubilizing effects of combined use of strain PSBHY-3 and De were studied.【Result】Compared with the control group,the total phosphorus(TP)content in the bottom sediment of the J-3(10^3CFU/mL),J-4(10^4 CFU/mL),J-5(10^5 CFU/mL)and J-6(10^6 CFU/mL)groups were significantly reduced(P<0.05,the same below),and the corresponding TP degradation rates were 7.05%,6.60%,4.03%and 9.17%respectively.In the experiment of combined use of PSBHY-3 and De,two strains not only did not show growth antagonism,but also promoted the growth of De.At the end of the experiment(the 8th d),the degradation rate of TP in the sediment was significantly higher in each addition group than that in the control group.Among them,TP degradation rate in group P was the highest(8.17%),while those in groups P-3D,P-D,D and 3P-D were 5.38%,4.97%,4.38%and 3.91%respectively.There was no significant difference among each addition group(P>0.05).The strain PSBHY-3 was identified as Bacillus amylliqueficiens by 16S rDNA sequence analysis and Biolog microbiological identification system.【Conclusion】Strain PSBHY-3 is B.amylliqueficiens,which has a good degradation effect on TP in the bottom sediment of pond.And it can be used as an alternative strain of the probiotics of phosphate solubilizing bacteria applied in aquaculture.
作者
胡晓娟
许云娜
胡百文
徐煜
杨铿
文国樑
李卓佳
曹煜成
HU Xiao-juan;XU Yun-na;HU Bai-wen;XU Yu;YANG Keng;WEN Guo-liang;LI Zhuo-jia;CAO Yu-cheng(South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences/Key Laboratory of Fishery Ecology and Environment of Guangdong/Key Laboratory of South China Sea Fishery Resources Exploitation&Utilization,Ministry of Agriculture and Rural Affairs,Guangzhou 510300,China)
出处
《南方农业学报》
CAS
CSCD
北大核心
2018年第10期2096-2102,共7页
Journal of Southern Agriculture
基金
广东省科技计划项目(2016A020210024)
国家现代农业(虾蟹)产业技术体系建设专项项目(CARS-48)
中国水产科学研究院基本科研业务费专项项目(2017HY-ZD0501)
广东省渔港建设和渔业发展专项项目(A201701B06)
关键词
解磷菌
解磷效果
池塘底泥
有机物降解菌
复配使用
phosphate solubilizing bacteria
phosphorus removal effect
pond sediment
organic degrading bacteria
combined use
作者简介
胡晓娟(1984-),博士,主要从事养殖水环境与微生物调控研究工作,E-mail:xinr129@163.com;通讯作者,曹煜成(1979-),博士,副研究员,主要从事水产健康养殖和养殖生态环境调控修复研究工作,E-mail:cyc_715@163.com。
