摘要
试验旨在对筛选出的6种瘤胃源益生菌进行小鼠试验,以期为其在畜禽养殖业的进一步开发和利用提供参考依据。选取5周龄雄性昆明小鼠56只,随机分为7组:生理盐水对照组、解淀粉芽孢杆菌Lw-J21组、枯草芽孢杆菌Lw-K16组、乳酸菌Lw-R35组、产蛋白酶菌Lw-D48组、产纤维素酶菌Lw-X21组和酵母菌Lw-JM70组,试验组每天以0.2mL/10g体重剂量连续灌胃42d,对照组灌喂相同剂量的生理盐水,比较并分析灌胃不同益生菌对小鼠体重、肠道菌群数量、脂肪积累量、免疫器官指数和血糖降解能力等的影响。结果显示,与对照组相比,解淀粉芽孢杆菌Lw-J21和产纤维素酶菌Lw-X21组小鼠体重增加明显(P>0.05),同时可显著减少小鼠体内的脂肪积累(P<0.05)。乳酸菌Lw-R35和产纤维素酶菌Lw-X21组小鼠肠道中的乳酸菌和总厌氧菌数量均显著或极显著增多(P<0.05;P<0.01)。各益生菌组小鼠脾脏指数和胸腺指数均有显著或极显著提高(P<0.05;P<0.01),且可不同程度提高小鼠血糖的调节能力。综上所述,用筛选出的6种天然益生菌饲喂小鼠可改善其肠道微生态环境、免疫器官指数和血糖调节能力等。
The experiments of six kinds of probiotics screened from the rumen were conducted to provide references for their further development and utilization in livestock and poultry husbandry.56 male 5-week-old Kunming mice were chosen and randomly divided into 7 groups.The mice in control group was fed with 0.2 mL of normal saline,while that of the other experimental groups were fed with 0.2 mL/(10 g·BW)of Bacillus amyloliquefaciens Lw-J21,Bacillus subtilis Lw-K16,Lactobacillus Lw-R35,Bifidobacterium Lw-D48,cellulose-producing bacteria Lw-X21,Candida dattila Lw-JM70(1×10 7 CFU/mL)for 42 d,respectively.The effects of different probiotics on body weight,intestinal flora,fat accumulation,immune indexes and hypoglycemia of mice were compared and analyzed.The results showed that compared with the control group,the body weight of mice in Lw-J21 and Lw-X21 groups were higher(P>0.05),and the fat accumulation in mice were significantly reduced at the same time(P<0.05).The number of Lactobacillus and total anaerobic bacteria in the intestinal tract of mice in Lw-R35 and Lw-X21 groups were significantly or extremely significantly increased(P<0.05;P<0.01).The spleen index and thymus index of mice in probiotics groups significantly or extremely significantly enhanced(P<0.05;P<0.01),meanwhile the hypoglycemia of mice was improved.In conclusion,the screed probiotics had different degrees of influence on intestinal microenvironment,immune indexes and blood glucose regulation ability of mice.
作者
冀海龙
王红英
周英男
钱斯日古楞
JI Hailong;WANG Hongying;ZHOU Yingnan;QIAN Siriguleng(College of Biological Engineering,Dalian Polytechnic University,Dalian 116034,China)
出处
《中国畜牧兽医》
CAS
北大核心
2018年第9期2463-2470,共8页
China Animal Husbandry & Veterinary Medicine
基金
内蒙古自治区科技重大专项项目(222089)
关键词
益生菌
肠道菌群
脂肪积累量
免疫器官指数
血糖
probiotic
intestinal flora
fat accumulation
immune organ indexes
blood glucose
作者简介
冀海龙(1990-),男,山西忻州人,硕士生,研究方向:工业微生物资源与生物催化,E-mail:185010321@qq.com;通信作者:钱斯日古楞(1969-),男,蒙古族,内蒙古兴安盟人,博士,教授,研究方向:微生物学,E-mail:srgl2000@163.com。
