摘要
本研究选用一株具有除草活性的生防菌株极细链格孢Alternariatenuissima HZ-1作为研究对象,通过载体和助剂的筛选,初步研制出可湿性粉剂,并对杂草离体叶片、盆栽杂草及田间小区杂草的致病效果进行了测定。结果表明,HZ-1最适载体为高岭土,助剂为羧甲基纤维素、可溶性淀粉和白炭黑,菌株HZ-1可湿性粉剂对不同杂草离体叶片致病性表现为:密花香薷>冬葵>野燕麦>薄蒴草>酸模叶蓼>藜>萹蓄。盆栽致病性结果显示,HZ-1可湿性粉剂对薄蒴草、藜和密花香薷致病效果突出,对冬葵和酸模叶蓼次之,对萹蓄、野燕麦无效。小区试验显示对繁缕、藜和密花香薷有显著除草效果。该菌株可作为防除薄蒴草、藜、密花香薷和繁缕等杂草的生物除草剂进行进一步研究。
Alternaria tenuissima HZ-1 was selected as a high virulent strain to weeds.Proper carrier and auxiliary were screened and wettable powder was developed as biological herbicide,and the virulence to different weeds in vitro and in vivo was also determined.The results showed that the optimal vector of HZ-1 was kaolin,the auxiliarges were carboxymethyl cellulose,soluble starch and silica.The susceptibility of strain HZ-1 wet powder to different weeds in vitro was as follows:Elsholtzia densa Benth.>Malva verticillata L.var.crispa>Avena fatua L.>Lepyrodiclis holosteoides(C.A.Mey.)Fisch.et Mey.>Polygonum lapathifolium L.>Chenopodium album L.>Polygonum aviculare L..Pot experiments showed that wet powder of HZ-1 had significant virulence to L.holosteoides,C.album and E.densa,but no virulence to P.aviculare and A.fatua.Field trials demonstrated that wet powder of HZ-1 was highly virulent to Stellaria media,C.album and E.densa.This strain could be further studied as a biological herbicide to control L.holosteoides,C.album,E.densa and Stellaria media.
作者
朱海霞
马永强
郭青云
ZHU Haixia;MA Yongqiang;GUO Qingyun(Academy of Agriculture and Forestry Sciences of Qinghai University,State Key Laboratory of Plateau Ecology and Agriculture of Qinghai University,Scientific Observing and Experimental Station of Crop Pest in Xining,Ministry of Agriculture,Key Laboratory of Agricultural Integrated Pest Management in Qinghai Province,Xining 810016,China)
出处
《植物保护》
CAS
CSCD
北大核心
2018年第5期212-216,230,共6页
Plant Protection
基金
国家自然科学基金(31560518)
中国科学院“西部之光”人才培养引进计划(201523)
关键词
极细链格孢菌HZ-1
菌剂研制
除草效果
Alternaria tenuissima HZ-1
development of agent
control effect to weeds
作者简介
为并列第一作者:朱海霞;为并列第一作者:马永强;通信作者,郭青云,E-mail:guoqingyunqh@163.com
