摘要
本实验测定了5个产地茶花粉的总酚和总黄酮含量,在此基础上,研究了茶花粉的体外抗氧化活性及其对酒精诱导的急性肝损伤的保护作用。结果表明,浙江茶花粉的总酚和总黄酮含量最高,分别为8.97 mg/g和3.01 mg/g,并且具有较高的抗氧化活性。给小鼠连续灌胃茶花粉7周,小鼠血清氧自由基吸收能力显著增加(P<0.05),Cu2+诱导的脂质过氧化损伤显著改善(P<0.05)。与模型组相比,茶花粉组小鼠血清中谷草转氨酶和谷丙转氨酶活力显著下降(P<0.05),肝脏组织中超氧化物歧化酶和谷胱甘肽过氧化物酶活力显著上升(P<0.05),丙二醛含量显著降低(P<0.05)。组织病理学观察显示,茶花粉能够明显改善酒精引起的肝细胞损伤。本研究结果表明茶花粉不仅具有较强的抗氧化活性,还对酒精造成的急性肝损伤有明显的保护作用,可以作为一种保护肝脏损伤的功能性食品原料。
In the present study,Camellia japonica bee pollen from five geographical origins in China were analyzed for total phenolic and total flavonoid contents.We also evaluated the antioxidant capacity and hepatoprotective effects of C.japonica bee pollen on acute alcohol-induced liver damage in mice.The results showed that C.japonica bee pollen from Zhejiang province exhibited the highest total phenolic(8.97 mg/g calculated as gallic acid equivalent)and total flavonoid content(3.01 mg/g calculated as rutin equivalent)and strong antioxidant capacity.Oral administration of mice with crude C.japonica bee pollen for 7 consecutive weeks significantly increased serum oxygen radical absorbance capacity(P<0.05),lowered the levels of aspartate aminotransferase and alanine aminotransferase as well as lipoprotein oxidation in serum(P<0.05),enhanced the activities of glutathione peroxidase and superoxide dismutase in liver(P<0.05),and decreased the formation of malondialdehyde in liver(P<0.05).Histopathological examinations showed that C.japonica bee pollen is efficacious for the prevention and treatment of alcohol-induced hepatic injury in mice.This study concluded that C.japonica bee pollen exhibits hepatoprotective properties and may be used as an ingredient of functional foods.
作者
彭国霞
赵浩安
刘清清
张颖
程妮
曹炜
PENG Guoxia;ZHAO Haoan;LIU Qingqing;ZHANG Ying;CHENG Ni;CAO Wei(College of Food Science and Engineering,Northwest University,Xi’an 710069,China;Bee Product Research Center of Shaanxi Province,Xi’an 710065,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2018年第17期127-133,共7页
Food Science
基金
国家自然科学基金面上项目(31272510)
陕西省社会发展攻关项目(2016SF-425)
西安市农业技术研发项目(NC1405(1))
陕西省教育厅产业化培育项目(16JF025)
关键词
茶花粉
总酚
总黄酮
抗氧化
保护肝脏
Camellia japonica bee pollen
total polyphenols
total flavonoids
antioxidant
hepatoprotective
作者简介
彭国霞(1992—),女,硕士研究生,研究方向为功能性食品。E-mail:pengguoxia@stumail.nwu.edu.cn.;通信作者简介:曹炜(1969—),男,教授,博士,研究方向为蜂产品深加工及质量控制。E-mail:caowei@nwu.edu.cn.
