摘要
采用水蒸气蒸馏法提取来自广西尾巨桉(Eucalyptus urophylla×E.grandis)不同杂种无性系叶片的挥发性成分,并通过气相色谱-质谱(GC-MS)、气相色谱(GC)分析其挥发性成分种类及含量。结果表明,杂交无性系DH42-6、DH19-6、DH32-26和DH32-29分别检测出34、34、30和32种化学物质。其中,挥发性成分超过1%以上的化学成分分别有7、8、5和7种,分别占总挥发性成分的91.418%、89.598%、95.420%和92.676%。按主要挥发性成分分类,这4种尾巨桉杂交无性系可分为两种生化类型,一种为1,8-桉叶素型,主要产生的无性系为DH19-6和DH32-26;另一种为γ-松油烯型,主要产生的无性系为DH42-6和DH32-29。不同无性系叶片主要成分的差异导致其萜类化合物及其衍生物的主要含量有所差异。DH19-6和DH32-26叶片挥发性成分的单萜醇酯类物质为主要成分,其相对百分含量分别为49.973%和46.290%;DH42-6和DH32-29叶片挥发性成分中的单萜类物质为主要成分,其相对百分含量分别达到91.010%和83.745%。尾巨桉不同杂种无性系叶片挥发性成分的差异,为后续研究桉树化感物质的化感作用提供树种来源及理论基础。
Volatile components from leaves of different hybrid clones of Eucalyptus urophylla×E.grandis from Guangxi were extracted by steam distillation and volatile components were analyzed by gas chromatogra?phy-mass spectrometry(GC-MS)as well as gas chromatography(GC).Results showed that 34,34,30 and 32 chemical substances were detected in the hybrid clones DH42-6,DH19-6,DH32-26 and DH32-29,respectively.Among them,there are 7,8,5 and 7 chemical components with volatile compo?nents exceeding 1%,accounting for 91.418%,89.598%,95.420%and 92.676%of the total volatile compo?nents.According to the main volatile components,the four hybrid clones of E.urophylla×E.grandis can be divided into two kinds of biochemical types.One was 1,8-cineole type,which mainly produced by DH19-6 and DH32-26;the other isγ-terpinene type,which mainly produced by DH42-6 and DH32-29.Differenc?es in the main components of the leaves of different clones led to the differences in the main contents of terpe?noids and their derivatives.Among them,the monoterpene esters were the main volatile components of DH19-6 and DH32-26,and the percentage composition was 49.973%and 46.290%,respectively.The monoterpenoids in the volatile components of DH42-6 and DH32-29 leaves were dominant components and the percentage composition reached 91.010%and 83.745%.The difference of volatile components in leaves of different hybrid clones of E.urophylla×E.grandis could provide the tree species and theoretical basis for the subsequent study on allelopathy of Eucalyptus allelochemicals.
作者
黄妹平
陈风帆
廖仲秋
梁忠云
张照远
HUANG Mei-ping;CHEN Feng-fan;LIAO Zhong-qiu;LIANG Zhong-yun;ZHANG Zhao-yuan(Guangxi Forestry Research Institute,Guangxi Key Laboratory of Superior Timber Trees Resource Cultivation,Key Laboratory of Central South Fast-growing Timber Cultivation of Forestry Ministry of China,Nanning 530002,China)
出处
《广西林业科学》
2018年第2期165-169,共5页
Guangxi Forestry Science
基金
广西创新驱动发展专项(桂科AA17204087-3)
广西自然科学基金(2014GXNSFBA118107)
关键词
尾巨桉
杂种无性系
挥发性成分
水蒸气提取法
气相色谱-质谱
气相色谱
Eucalyptus urophylla×E.grandis
hybrid clones
volatile component
extraction by steam ex.traction
gas chromatography-mass spectrometry(GC-MS)
gas chromatography(GC)
作者简介
第一作者:黄妹平(1989—),硕士,助理工程师,主要从事林木遗传育种研究。;通信作者:张照远(1980—),博士,高级工程师,主要从事林木遗传育种研究。
