摘要
目的观察滋肾育胎丸(菟丝子、人参、续断,等)对复发性流产(RSA)小鼠的大鼠肉瘤蛋白(RAS)、血管内皮细胞生长因子(VEGF)、血管内皮细胞生长因子受体2(VEGFR-2)、可溶性血管内皮生长因子受体-1(s VEGFR-1)、丝裂原活化蛋白激酶(MAPK)等因子的调节作用,探讨其作用机制。方法分别选取CBA/J雌性小鼠和DBA/2雄性小鼠,CBA/J雌性小鼠与BALB/c雄性小鼠,按雌雄2∶1比例合笼交配,以检出阴栓者计为妊娠第0天,分别建立得到RSA模型和正常妊娠模型CBA×BALB/c小鼠;取造模成功的CBA/J×DBA/2小鼠30只,随机分为模型对照组、滋肾育胎丸组和黄体酮胶囊组,10只CBA×BALB/c小鼠作为正常妊娠对照组。各给药组连续给药15 d后,检测并计算各组小鼠子宫胚胎丢失率,HE染色观察小鼠蜕膜组织病理变化,免疫组化和real-time PCR分别检测RAS、VEGF、VEGFR-2、s VEGFR-1、MAPK的蛋白和mRNA表达。结果滋肾育胎丸能够显著降低RSA小鼠的胚胎丢失率,改善RSA小鼠蜕膜组织的病理改变,能够通过降低RAS、VEGF、VEGFR-2的水平和提高s VEGFR-1的表达,调节小鼠蜕膜组织的血管生成及重铸。结论滋肾育胎丸能够通过调节RAS、VEGF、VEGFR-2及s VEGFR-1等因子的表达,改善RSA小鼠胚胎丢失率和蜕膜血管新生。
AIM To observe the effect of Zishen Yutai Pills(Cuscutae Semen,Ginseng Radi%et Rhizoma,Dipsaci Radix,etc.)on RAS,VEGF,VEGFR-2,sVEGFR-1 and MAPK in recurrent miscarriage mice,and to explore its mechanism.METHODS CBA/J female mice+DBA/2 male mice,and CBA/J female mice+BALB/c male mice were mated by 2 females and 1 male in cage to establish the RSA model and the normal pregnancy CBA x BALB/c mouse model respectively.Since the zeroth day of pregnancy,a total of 24 CBA/J x DBA/2 mice were randomly divided into model control group,Zishen Yutai Pills group and progesterone capsule group,and 10 CBA x BALB/c mice were used as normal pregnancy control group.Mice of all groups after the respective 15-day intervention had their rate of uterine embryo loss measured and calculated.Their pathological changes of decidual tissue were determined by HE staining,their RAS,VEGF,VEGFR-2,sVEGFR-1,MAPK protein and mRNA expressions were detected by immunohistochemistry and real-time PCR.RESULTS Zishen Yutai Pills significantly reduced the rate of embryo loss and improved pathological changes of decidual tissue in RSA mice through regulating mouse decidual tissue angiogenesis and recasting,as revealed by the lowered levels of RAS,VEGF,VEGFR2 and MAPK,and increased expression of sVEGFR-1.CONCLUSION Zishen Yutai Pills can lower the rate of embryo loss and improve decidual angiogenesis in RSA mice through altering the expression of RAS,VEGF,VEGFR-2,sVEGFR-1 and MAPK.
作者
储继军
王瑞雪
余欣慧
刘会会
李伟莉
韩燕全
CHU Ji-jun;WANG Rui-xue;YU Xin-hui;LIU Hui-hui;LI Wei-li;HAN Yan-quan(The First Hospital Affiliated to Anhui University of Chinese Medicine,Hefei 230031,China)
出处
《中成药》
CAS
CSCD
北大核心
2018年第4期777-782,共6页
Chinese Traditional Patent Medicine
基金
罗元恺滋肾育胎丸中青年科研基金项目(140206)
关键词
滋肾育胎丸
复发性流产
RAS蛋白
血管内皮生长因子
Zishen Yutai Pills
recurrent spontaneous abortion
RAS protein
vascular endothelial growth factor
作者简介
储继军(1977—),男,博士,副主任医师,研究方向为中医药防治自然流产、不孕不育症等。Tel:13305694773,E-mail:280711035@qq.com;通信作者:李伟莉(1958—),女,教授,主任医师,硕士生导师,研究方向为中医药防治自然流产、不孕不育症等。Tel:13075596906,E-mail:liweiliah@163.com
