摘要
病程相关蛋白(Pathogenesis-related protein,PR)是参与植物抗病性的重要物质,在诱导系统抗性过程中起着重要作用。本研究以青花菜为材料,在克隆BoPR1基因的基础上,利用荧光定量PCR技术研究它们在根肿菌和核盘菌侵染下的表达模式。序列分析结果表明,BoPR1基因组全长为489bp,无内含子,编码162个氨基酸,具1个信号肽和1个SCP结构域。系统发育分析的结果表明,BoPR1与甘蓝型油菜和白菜的PR1遗传距离最小,亲缘关系最近,在进化树上聚为一组;与醉蝶花PR1遗传距离最大,亲缘关系最远。荧光定量PCR结果显示,BoPR1基因的表达受根肿菌诱导,在接种5d时的表达量最高,为对照的11.84倍;BoPR1基因的表达则不受核盘菌诱导。
Pathogenesis-related proteins(PRs)are crucial in the induced systemic disease resistance for plants.Using real-time fluorescence quantitative PCR,this study isolated a gene,designated as BoPR1,frombroccoli to examine its expression patterns after inoculated by pathogens,Plasmodiophora brassicae and Sclerotinia sclerotiorum.A sequence analysis indicated that the full genome DNA of BoPR1 was 489 bp in length encoding 162 amino acids and containing no intron.The deduced protein consisted of a signal peptide and a SCP domain.The results from aphylogenetic analysis showed that BoPR1 had a minimum genetic distance,clustering on a same clade,with the PR1 proteins from Brassica napus and B.rapa,indicating a close relationship among them.On the other hand,it was remotely related to Tarenaya hassleriana,as the genetic distance between the two PR1swas the greatest among the tested samples.Theq RT-PCR results suggested that the expression of BoPR1 was induced by P.brassicae,with the highest level observed 5 d after inoculationwhich was 11.84-fold of control.But,BoPR1 expression was not affected by S.sclerotiorum.Theobtained isolation and expression information on BoPR1 would be useful for futureresearch on thedisease resistance mechanism as well as the molecular breeding programs on B.oleracea.
作者
何佳
葛露霞
金魏佳
范灵希
章燕如
叶佳燕
郑颖
蒋明
HE Jia;GE Lu-xia;JIN Wei-jia;FAN ling-xi;ZHANG Yan-ru;YE jia-yan;ZHENG Ying;JIANG Ming(College of Life Sciences,Taizhou University,Jiaojiang,Zhejiang 318000,China)
出处
《福建农业学报》
CAS
北大核心
2018年第1期35-40,共6页
Fujian Journal of Agricultural Sciences
基金
浙江省大学生新苗人才计划(2017R430012)
台州市科技计划项目(162ny14)
浙江省公益性技术应用研究计划项目(2016C32091)
关键词
青花菜
BoPR1
基因克隆
表达分析
Brassica oleracea var.italica
BoPR1
gene cloning
expression analysis
作者简介
何佳（1995-），女，本科生，研究方向：植物分子生物学（E-mail：1692790364@qq.com）;通讯作者：蒋明（1973-），男，博士，副教授，研究方向：植物逆境生物学及其分子调控（E-mail：jiangming1973@139.com）
