摘要
【目的】研究Box-Behnken(BBK)试验设计结合响应面分析法提高玉米秸秆的降解率。【方法】从玉米秸秆堆肥的盐碱土壤中分离筛选出4株高效耐盐纤维素降解菌WH2、WH4、WH7和WH9,经形态观察、生理生化反应和分子鉴定,确定4株菌分别为蜡样芽孢杆菌(Bacillus cereus)、荧光假单胞菌(Pseudomonas formosensis)、枯草芽孢杆菌(Bacillus subtilis)和松嫩假单胞菌(Pseudomonas songnenensis)。在单因素试验、BBK中心组合试验设计及响应面法分析法的基础上,以玉米秸秆降解率为响应值,利用响应面法研究各因素及其交互作用对玉米秸秆降解率的影响,优化菌种配比工艺。【结果】耐盐纤维素降解菌的最佳配比工艺:当秸秆添加量为2.0 g时,菌种WH2、WH4、WH7和WH9接种量分别为0.31、0.21、0.32和0.22 g时的玉米秸秆降解率达到最大值。【结论】在耐盐纤维素降解菌最优配比条件下,玉米秸秆降解率预测值为61.17%,验证值为60.90%,两值相差0.27%,证明了响应面法的合理性和有效性,为新疆盐碱地区玉米秸秆的微生物降解提供科学依据。
【Objective】To increase the degradation rate of maize straw by employing response surface method based on Box-Behnken(BBK).【Method】Four salt tolerant strains(WH2,WH4,WH7and WH9)with good ability of degrading cellulose were isolated from saline alkali soil of corn straw manure.Based on morphologic observation,physiological and biochemical characteristics and molecular identification,they were identified as Bacillus cereus,Pseudomonas formosensis strain,Bacillus subtilis and Pseudomonas songnenensis respectively.On the basis of single-factor test analysis,Box-Behnken central composite design and response surface method(RSM),while taking the degradation rate of maize straw as the response value,response surface method was applied to analyze the influence of various factors and their interaction on the degradation rate of maize straws,thus optimizing the progress ratio of bacteria.【Result】The result showed that the optimum conditions were as follows:When the straw is2.0g,the inoculation quantity of the four strains(WH2,WH4,WH7,WH9)is0.31g,0.21g,0.32g and0.22g,respectively,and the rate of straw degradation reached the maximum.【Conclusion】Under these optimal conditions,the predictive value of the degradation rate of straw is61.17%,meanwhile the verification value is60.90%.There is only0.27%difference between predictive and verification value,which has proved the rationality and effectiveness of RSM.The experiment has provided a theoretical basis for the microbial degradation of corn straws in Xinjiang.
作者
王旭辉
叶凯
徐鑫
山其米克
王卉
李冠
邓宇
WANG Xu-hui;YE Kai;XU Xin;Shanqimike;WANG Hui;LI Guan;DENG Yu(College of Life Sciences and Technology,Xinjiang University,Urumqi 830046,China;Organisms Energy Research Institute of Xinjiang Academy of Agriculture Sciences,Urumqi 830091,China;Research Institute of Food Crops,Xinjiang Academy of Agricultural Sciences, Urumqi 830091,China;Biogas Research Institute of Ministry of Agriculture,Chengdu Sichuan,610041,China)
出处
《新疆农业科学》
CAS
CSCD
北大核心
2017年第12期2282-2292,共11页
Xinjiang Agricultural Sciences
基金
新疆维吾尔自治区科研机构创新发展专项资金项目"乌鲁木齐南郊近山带循环农业关键技术研究"(2016D04019)
农业部农村可再生能源开发利用重点实验室开放基金"耐盐纤维素降解菌的筛选及发酵工艺优化"(2017007)
新疆农业科学院院青年基金项目"生物预处理对沼气生产原料及产气影响"(xjnky-2013021)~~
关键词
玉米秸秆
降解率
菌种鉴定
响应面法
corn straw
strains identification
degeneration rate
response surface methodology
作者简介
王旭辉(1985-),男,新疆人,助理研究员,博士研究生,研究方向为生物质能源利用,(E-mail)bio0407015@qq.com;通讯作者: 李冠(1949-),男,湖北人,教授,博士生导师,研究方向为植物生理生化与分子生物学,(E-mail)guanli@xju.edu.cn;通讯作者:邓宇(1963-),男,四川人,教授,博士生导师,研究方向为沼气微生物,(E-mail)dengyu@caas.cn
