摘要
目的探讨小鼠血管平滑肌细胞(vascular smooth muscle cells,VSMCs)在机械牵张力(stretch stress,SS)和氧化低密度脂蛋白(oxidized low-density lipoprotein,ox LDL)作用下全基因组甲基化水平的变化,并进一步考察辛伐他汀(simvastatin,SIM)对此变化的影响。方法血浆制备氧化型低密度脂蛋白,体外常规培养VSMCs,施加机械牵张力和/或氧化型低密度脂蛋白,或用辛伐他汀预处理1h后再施加刺激,用荧光探针5-甲基胞嘧啶检测VSMCs全基因组甲基化水平。以细胞免疫荧光定量阳性率和SPSS统计软件对甲基化水平进行分析。结果机械牵张力和氧化型低密度脂蛋白均可降低VSMCs的全基因组甲基化水平,且两者联合作用大于单一因素,辛伐他汀预处理VSMCs可部分抑制机械牵张力和/或氧化低密度脂蛋白诱导的全基因组甲基化水平降低。结论机械牵张力和氧化低密度脂蛋白可分别促进VSMCs全基因组甲基化水平降低,当两者共处理时呈相加作用,辛伐他汀可部分抑制上述效应。
Objective To investigate genome-wide methylation in mouse vascular smooth muscle cells(VSMCs)induced bystretch stress(SS)and oxidized low-density lipoprotein(oxLDL),and the role of simvastatin in this process.Methods VSMCs weretreated with SS and/or oxLDL prepared from plasma with or without pre-incubation of simvastatin for60min.The methylation wasdetected using5-methylcytosine fluorescent probe.The level of methylation was determined by fluorescence positive rate which wasquantified using SPSS analysis.Results Either SS or ox-LDL decreased the genome-wide methylation of VSMCs.The combination ofboth caused more significant reduction.Simvastatin partly inhibited the effect induced by SS and/or oxLDL.Conclusion Both SS andox-LDL can reduce genome-wide methylation in VSMCs independently,and to a higher degree when applied together.Simvastatincan partly abolish this effect.
作者
刘科峰
裴婷
刘树迎
李子卿
平苏宁
王晶晶
汪泓
盛璞义
李朝红
Liu Kefeng;Pei Ting;Liu Shuying;Li Ziqing;Ping Suning;Wang Jingjing;Wang Hong;Sheng Puyi;Li Chaohong(Department of Histology and Embryology, Xiangnan University, Chenzhou 423000, China;Department of Histology and Embryology, Zhongshan School of Medicine,Sun Yat-sen University, Guangzhou 510080, China;Departments of orthopedic Surgery,Sun Yat-sen University First Affiliated Hospital, Guangzhou 510080,China)
出处
《中国组织化学与细胞化学杂志》
CAS
CSCD
2017年第4期360-365,共6页
Chinese Journal of Histochemistry and Cytochemistry
基金
国家自然科学基金项目(81070124
81500337)
广东省科技计划项目(2014A020212109)
湖南省教育厅资助科研项目(12C0893)
湖南省重点建设学科资金资助(湘教发[2011]76号)
作者简介
刘科峰，女（1978年），汉族，博士;通讯作者：lichaoh@mail.sysu.edu.cn
