摘要
以灰树花菌株QY-01为材料,在添加不同种类及浓度植物生长调节剂的培养基上培养,以灰树花的菌落直径为主要指标。研究植物生长调节剂对灰树花菌丝生长的影响规律,筛选促进灰树花菌丝生长的植物生长调节剂及其适宜使用浓度。结果表明,KT-30和6-BA对白灵菇菌丝生长的促进作用强,芸苔素和赤霉素对其菌丝生长无作用,NAA、三十烷醇、DA-6等的促进作用较弱;菌丝对6-BA非常敏感,对NAA表现较强耐受性,高浓度的KT-30和6-BA导致菌落形态改变;KT-30、6-BA、NAA、三十烷醇、鲜胺酯、IAA、低聚壳聚糖、四甲基戊二酸和复硝酚钠对菌落直径的提高比例分别为57.9%、52.4%、11.7%、10.9%、10.2%、10.0%、8.5%、8.4%和8.1%,最佳浓度分别为4.9、10.0、5.0、0.1、3.7、3.6、7.5、6.5mg/L和2.7mg/L,对灰树花的液体发酵及液体菌种培养具有重要意义。
Grifola frondosastrain QY-01 was cultivated on the media supplemented with different types and concentrations of plant growth regulators to examine their effects on strain growth and to select optimal regulator and concentration by using colony diameter as index.The results showed that KT-30 and 6-BA had obvious promoting effect on the mycelium growth,while NAA,triacontanol,and DA-6were weaker.Canola and GA3 had no effect.The stain was very sensitive to 6-BA,and showed strong tolerance to NAA.High concentrations of KT-30 and triacontanol caused changes in the colony morphology.Compared with controls,the colony diameters of KT-30,6-BA,NAA,Triacontanol,DA-6,IAA,Chitosan,Tetramethyl gluttaric aciduria and Sodium nitrophenolate treated sample significantly increased by 57.9%,52.4%,11.7%,10.9%,10.2%,10%,8.5%,8.4% respectively,the corresponding optimal concentrations were4.9,10.0,5.0,0.1,3.7,3.6,7.5,6.5mg/L and 2.7mg/L,respectively.The result was of great significance to liquid fermentation and the strains cultivation of G.frondosa.
出处
《西北林学院学报》
CSCD
北大核心
2016年第2期188-194,共7页
Journal of Northwest Forestry University
基金
"十二五"科技支撑项目"西北地区板栗高效生产关键技术研究与示范"(2013BAD14B0404)
国家林业局重点推广课题"板栗新品种栽培技术集成与示范推广"[2014]44号
关键词
灰树花
植物生长调节剂
菌落直径
Grifola frondosa
plant growth regulator
colony diameter
