摘要
本研究旨在获得伏马毒素B1特异性适配体并利用其和胶体金的作用建立伏马毒素B1的快速检测方法。通过非固定靶标的指数富集配体系统进化技术筛选获得伏马毒素B1的适配体,并用酶联免疫吸附测定(ELISA)方法测定所获适配体的亲和力和特异性,建立基于适配体的胶体金快速定量检测方法。结果表明:筛选得到的伏马毒素B1适配体F102亲和力解离常数为(42.1±4.0)nmol/L;建立的胶体金检测方法简便、快速、特异性强;样品检测结果与液相色谱结果的相对误差低于15.7%。因此,本研究利用筛选得到的适配体建立了可用于谷物和饲料中伏马毒素B1含量的快速定量检测方法。
This experiment was conducted to obtain fumonisin B1 aptamers and to develop a rapid test method based the effect of fumonin B1 and colloidal gold. Used the systematic evolution of ligands by exponential enrichment technology of non-immobilized target to screen the fumonisin B1 aptamers,whose affinity and specificity was determined by enzyme-linked immunosorbent assay（ ELISA） method,and to develop a colloidal gold rapid and quantitative test based on aptamers. The results showed that the affinity dissociation constant of the aptamer F102 of fumonisin B1 was（ 42. 1 ± 4. 0） nmol/L,and the fumonisin B1 colloidal gold detection method was simple,rapid and specific. The relative error of fumonisin B1 colloidal gold detection results and high performance liquid chromatography detection results was less than 15. 7%. Therefore,the fumonisin B1 aptamers screened by the experiment can be used for testing the fumonisin B1 content in cereals and feedstuffs rapidly and quantitative.
作者
周妍
刁晨曦
张圆圆
陆涛峰
赵丽丽
陈洪岩
ZHOU Yan;DIAO Chenxi;ZHANG Yuanyuan;LU Taofeng;ZHAO Lili;CHEN Hongyan(College of Life Science,Northeast Agricultural University,Harbin 150030,China;Harbin Veterinary ResearchInstitute,Chinese Academy of Agricultural Sciences,State Key Laboratory of VeterinaryBiotechnology,Heilongjiang Provincial Key Laboratory of Laboratory Animal and ComparativeMedicine,Harbin 150069,China;College of Life Science and Technology,MudanjiangNormal University,Mudanjiang 157011,China)
出处
《动物营养学报》
CAS
CSCD
北大核心
2018年第9期3740-3750,共11页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
国家自然科学基金项目(31601974
31700140)
作者简介
周妍（1993-）,女,黑龙江哈尔滨人,硕士研究生,从事动物学研究.E-mail：2609814396@qq.com;通信作者：陈洪岩,研究员,E-mail：hychen@hvri.ac.cn
