摘要
目的:制备三氧化二砷脂质体,并建立测定三氧化二砷脂质体药物含量及包封率的方法。方法:通过醋酸铜梯度法制备三氧化二砷脂质体,利用银盐法测定砷的含量,采用葡聚糖凝胶微柱离心法分离游离药物与脂质体,并测定包封率。结果:确定银盐法检测砷的波长为510 nm,线性范围为4. 0～24. 0μg(r=0. 999 1),精密度、回收率符合要求。通过葡聚糖凝胶微柱离心可完全分离脂质体和游离药物。脂质体中砷含量为0. 296 mg·m L-1,包封率为83. 54%。结论:该方法准确度高、重复性好,适用于三氧化二砷脂质体的质量评价。
Objective： To prepare arsenic trioxide liposomes, and establish a method to determine the arsenic content and encapsulation efficiency of liposomes. Methods： The arsenic trioxide liposomes were prepared by copper acetate transmem- brane gradients. The content of arsenic was determined according to the silver diethyl-dithiocarbamate method. A sephadex micro- column centrifugation method was used for the separation of free arsenic trioxide from liposomes, and applied for the determination of encapsulation efficiency. Results： The determination wavelength was colffirmed to be 510 nm and the calibrated linear curve of elemental arsenic was in the range of 4.0-24.0 μg（ r =0. 999 l ）. The precision and recovery was accorded with the requirement of methodology. The free drug was completely separated from liposomes by centrifuging through sephadex microcol- umn. The content of arsenic in arsenic trioxide liposomes suspension was 0. 296 mg .mL-1 and the entrapment efficiency was 83. 54%. Conclusions： The method is accurate and reliable, which can be used to evaluate the quality of arsenic trioxide liposomes.
作者
刘春秀
王存杨
张斯杰
马佳
王绍宁
LIU Chun-xiu;WANG Cun-yang;ZHANG Si-jie;MA Jia;WANG Shao-ning(School of Pharmacy,Shenyang Pharmaceutical University,Shenyang 110016,China;School of Pharmaceutical Engineering,Shenyang Pharmaceutical U）liversity,Shenyang 110016,China;China National of Traditional ＆ Herbal Medicine Co.Ltd,Beijing 102600,China)
出处
《中国现代中药》
CAS
2018年第11期1427-1431,共5页
Modern Chinese Medicine
基金
辽宁省教育厅高等学校科学研究项目(L2014395)
关键词
三氧化二砷
脂质体
银盐法
包封率
葡聚糖凝胶微柱离心法
arsenic trioxide
liposomes
silver diethyl-dithio-earbamate method
encapsulation efficiency
sepha-dex mieroeolunm eentrifugation method
作者简介
[通信作者]王绍宁，副教授，研究方向：无机药物传递系统；Tel：（024）43520207，E-mail：wangsn_spu@163．com.
