摘要
目的建立测定利丙双卡因醇质体中利多卡因和丙胺卡因含量及包封率的高效液相色谱(HPLC)法。方法采用葡聚糖凝胶微柱离心法分离醇质体与游离药物,并通过HPLC法测定醇质体的包封率。采用Hypersil ODS2色谱柱(4.6 mm×150 mm,5μm),以0.05%磷酸二氢铵(p H7.0)-甲醇(30∶70,V/V)为流动相,流速为1.0 m L·min^(-1),检测波长为254 nm。结果利多卡因与丙胺卡因在10.00~100.0μg·m L^(-1)浓度范围内与峰面积呈良好的线性关系,r=0.999 5,回收率在98%~102%范围内(RSD<4%),3批醇质体中利多卡因包封率分别为82.46%,78.99%,80.73%;丙胺卡因包封率分别为84.04%,81.59%,84.13%。结论建立的微柱离心-高效液相色谱法便捷,重现性好,可用于测定利丙双卡因醇质体的包封率。
OBJECTIVE To establish a HPLC method for determination of the content and entrapment efficiency of lidocaine and prilocaine in compound ethosomes. METHODS Glucose gel minicolumn centrifugation was employed to separate the free drug from the ethosomes. The content and entrapment efficiency of lidocaine and prilocaine was determined by HPLC. Chromatographic conditions were as follows： column was Hypersil ODS2（ 4.6 mm × 150 mm,5 μm） and the mobile phase was 0. 05% ammonium dihydrogen phosphate（ pH= 7.0）-methanol（ 30 ∶ 70,V/V） with the flow rate of 1.0 m L·min^-1,and detection wavelength was 254 nm. RESULTS The calibration curve for lidocaine and prilocaine were linear in the range of 10.00-100.0 μg·m L^-1（ r= 0.999 5）.The average recovery were among 98%-102%（ RSD4%）. The entrapment efficiency of lidocaine in three ethosomes was 82.46%,78.99%,80.73% respectively.The entrapment efficiency of prilocaine in three ethosomes was 84. 04%,81. 59%,84. 13% respectively. CONCLUSION The established minicolumn centrifugation-HPLC method is simple,reproducible,and applicable for the determination of entrapment efficiency of lidocaine and prilocaine in compound ethosomes.
出处
《今日药学》
CAS
2017年第11期739-741,744,共4页
Pharmacy Today
基金
广东省医院药学研究基金(2016MZ03)
作者简介
邹国盛，主管药师，研究方向：医院药学，Tel：020-89168252，E-mail：593599130@qq．com;通信作者：何琳，高级实验师，研究方向：药物新剂型与新技术。Tel：020-39352176，E-mail：helin721@163．com
