摘要
目的探讨二甲双胍联合紫杉醇对人乳腺癌MCF-7细胞增殖的抑制作用。方法对人乳腺癌细胞MCF-7进行体外培养,分别以二甲双胍、紫杉醇、二甲双胍联合紫杉醇进行干预,通过CCK-8试剂盒测定干预48小时对人乳腺癌MCF-7细胞增殖的抑制情况。结果联合组细胞抑制率显著高于紫杉醇组和二甲双胍组(P_均<0.05)。联合组G1期细胞比例和Bax蛋白表达水平均显著高于二甲双胍组和紫杉醇组(P_均<0.05),S期、G2期细胞比例及磷酸化细胞外信号调节激酶、细胞外信号调节激酶、B细胞淋巴瘤/白血病-2蛋白表达水平均显著低于紫杉醇组和二甲双胍组(P_均<0.05)。结论二甲双胍联合紫杉醇可抑制人乳腺癌MCF-7细胞增殖,其机制可能与细胞丝裂原激活蛋白激酶通路调节有关。
Objective To investigate the inhibitory effect of Metformin combined with Paclitaxel on the proliferation of human breast cancer MCF-7 cells. Method Human breast cancer MCF-7 cells was cultured, Metformin, Paclitaxel, Metformin and Paclitaxel were used for intervention. The proliferation inhibition of MCF-7 cells was observed by CCK-8 kit for 48 hours. Result The cell inhibition rate of combined group was significantly higher thin1 that of Paclitaxel group and Metformin group （Pall 〈 0.05）. The proportion of G1 cells and Bax protein in combined group were significantly higher than that of Metformin group mid Paclitaxel group （Pall 〈0.05）, and the proportion of cells in S phase and G2 phase, the expression level of phosphorylation extracellulaJc signal-regulated kinase （p-ERK）, extracel-lular regulated protein （ERK）, and B cell lymphoma/leukemia-2 （Bcl-2） proteins were significantly lower than Paclitaxel group and Metformin group （Pall 〈 0.05）. Conclusion Metformin combined with Paclitaxel can inhibit the proliferation of human breast cancer MCF-7 cells, and its mechanism may be related to the regulation of mitogen-activation protein kinase pathway.
作者
梁君伟
吕喜英
方志华
LIANG Jun-wei;LYU Xi-ying;FANG Zhi-hua(Department of Oncology,Affiliated Hospital of Chengde Medical College,Hebei,Chengde 067000,China)
出处
《中国医学前沿杂志（电子版）》
2018年第11期70-73,共4页
Chinese Journal of the Frontiers of Medical Science(Electronic Version)
基金
河北省医学科学研究重点课题计划(ZL20140246)
作者简介
通讯作者：吕喜英E-mail：79818794@qq.com
