摘要
建立了高效液相色谱(HPLC)法测定甘蔗叶片样品中内源ABA含量的分析方法。以流动相甲醇-5%乙酸为缓冲液,等度洗脱,紫外检测波长为254 nm。通过实验条件优化,0.25~10 mg/L范围内ABA标准溶液检测结果呈现良好的线性关系(相关系数r2=0.996),加标回收率为87.5%~102.4%,相对标准偏差不大于5%,检出限LOD=0.0202 mg/L,定量限LOQ=0.0675 mg/L。结果表明该方法能达到定量检测的目的,可以用于甘蔗叶片中内源ABA含量检测。
High performance liquid chromatography （HPLC） method was used to test the ABA （Abscisic acid）content in sugarcane leaves. The sugarcane samples after grinding were extracted with methanol- acetic acid （5%）as mobile phases for isocratic elution. The detection wavelength was set at 254 nm with a UV-detector. With theoptimized conditions, the detection results of ABA standard solution in the range of 0. 25-10 mg/L showed a goodlinear relationship （Correlation coefficient r2=0.996）. The recoveries of samples reached 87.5%-102.4%, and therelative standard deviation was not more than 5%. The limit of detection （LOD） was 0.0202 mg/L, and the limit ofquantification （LOQ） was 0.0675 mg/L. The results showed that the method can achieve the purpose ofquantitative detection, which can be used to detect ABA content in sugarcane leaves.
作者
高欣欣
刘少春
刀静梅
方志存
邓军
樊仙
GAO Xin-xin, LIU Shao-chun, DAO Jing-mei, FANG Zhi-cun, DENG Jun, FAN Xian(Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences/Yunnan Key Laboratory of Sugarcane Genetic Improvement,Kaiyuan 661699, Yunna)
出处
《中国糖料》
2018年第6期30-32,共3页
Sugar Crops of China
基金
国家自然科学基金项目"甘蔗内源激素和主要酶系调控脱叶性的作用机理"(31160257)
关键词
甘蔗叶片
高效液相色谱
脱落酸含量
sugarcane leaves
high performance liquid chromatography （HPLC）
ABA content
作者简介
高欣欣（1986-）,女,助理研究员,硕士,从事甘蔗生理栽培研究.;通信作者：刘少春（1964-）,男,研究员,硕士,主要从事甘蔗生理栽培研究.E-mail：yn120712@163.com
