摘要
以红蜡蘑为试材,研究了液料比、超声功率、超声时间、超声温度、提取次数对多糖得率的影响,在单因素试验基础上,采用正交实验优化了工艺条件,对提取产物多糖进行了DPPH·、ABTS+·清除研究。结果表明:对多糖提取率影响最大的是提取次数,其次是液料比,提取时间影响最小;最佳提取工艺条件为提取3次,液料比20∶1mL·g^(-1),超声功率300 W,超声温度70℃,超声时间40 min,在此条件下多糖提取率达6.27%。多糖对DPPH·、ABTS+·有一定的清除作用,说明红蜡蘑多糖具有一定的抗氧化活性。
Taking Laccaria laccata as the raw material,the effects of extraction conditions such as ratio of liquid-solid,ultrasonic time,ultrasonic power,ultrasonic temperature and time of extraction were investigated by one-factor tests and orthogonal test,and the extraction polysaccharides were analyzed with DPPH· and ABTS＋· test.The results showed that the times of extraction affected the yield markably,the second was ratio of liquid-solid,and ultrasonic time had the least influence;the optimum coditions were determined as follows：times of extraction of 3 times,ratio of liquid-solid of20∶1 mL·g^-1,ultrasonic power of 300 W,ultrasonic temperature of 70 ℃ and ultrasonic time of 40 minutes.Under the condition,the yield was up to 6.27%.Polysaccharides exhibited antioxidant effects in 2,2′-diphenyl-1-picrylhydrazyl（DPPH·）,2,2′-azino-bis（3-ethylben-othiazoline-6-sulfonic acid（ABTS＋·）radical scavenging assay.And it showed that the extraction polysaccharides which had some oxidation resistance.
作者
张水花
李本鸿
周伟东
ZHANG Shuihua;LI Benhong;ZHOU Weidong(Faculty of Chemistry and Environmental Science,Qujing Normal College,Qujing,Yunnan 655011;Library,Qujing Normal College,Quj ing,Yunnan 655011)
出处
《北方园艺》
CAS
北大核心
2018年第17期133-138,共6页
Northern Horticulture
基金
云南省科技厅研究基金资助项目(2016FD081)
作者简介
张水花（1980-），女，硕士，副教授，研究方向为天然产物化学及保健食品。E-mail：z_shh@163．com
