摘要
为探究NAC转录因子在花生生长发育和抗逆反应中的功能,本试验在花生J11叶片中克隆得到一个NAC基因,并利用实时荧光定量PCR技术(RT-qRCR)对NAC基因表达模式进行了分析。结果表明,NAC基因全长1 752 bp,共编码583个氨基酸,分子量64.924 k Da,等电点4.42,亚细胞定位结果预测该NAC蛋白主要在细胞核中,进化树分析结果表明其与大豆GmNAC53亲缘关系较近,将其命名为AhNAC53。RT-qRCR结果表明,AhNAC53基因在花生不同组织中均有表达,其中在叶中的表达量最高;不同程度的干旱胁迫下其表达量存在显著差异,除在10%PEG6000胁迫下基因表达量相对变化较小,其他胁迫程度(5%、15%和20%PEG6000)下,随着处理时间的延长,AhNAC53基因表达量均呈现较大幅度的上调,且均在处理48 h时达到峰值,进一步表明AhNAC53基因可能参与了花生的生长发育和对干旱胁迫的响应。本试验结果为深入研究花生AhNAC53基因的功能奠定了一定的理论基础。
In order to explore NAC transcription function in peanut,we cloned a NAC gene from leaves of J11 peanut,used the real-time quantitative PCR（RT-qRCR） to analyze its expression patterns. This gene was 1 752 bp in length,encoding 583 amino acids with the molecular weight of 64. 924 k Da and the p I value of 4. 42,subcellular localization prediction has shown that this NAC protein was located in nucleus. Phylogenetic tree analysis demonstrated that it was closely related to Gm NAC53,so we named it AhNAC53. The RT-q PCR results showed that AhNAC53 expressed in different tissues of peanut,with the highest expression in leaves,and different drought stress could induce the significant difference in the expression of this gene. In addition to the relative small changes in gene expression under 10%PEG6000 stress,the relative expression levels of other stress（5%,15% and 20% PEG6000） were significantly increased with the prolongation of treatment time,and all reached the peak value at 48 h. These results indicated that AhNAC53 might play a significant role in peanut development and its response to drought stress,which laid the molecular basis for further studying its function.
作者
贾伟
李春娟
闫彩霞
赵小波
王娟
孔青
孙全喜
单世华
JIA Wei;LI Chunjuan;YAN Caixia;ZHAO Xiaobo;WANG Juan;KONG Qing;SUN Quanxi;SHAN Shihua(College of Food Science and Engineering,Ocean University of China,Qingdao,Shandong 266000;Shandong Peanut Research Institute,Qingdao,Shandong 266100)
出处
《核农学报》
CAS
CSCD
北大核心
2018年第10期1898-1907,共10页
Journal of Nuclear Agricultural Sciences
基金
国家国际科技合作专项项目(2015DFA31190)
山东省农业良种工程项目
山东省现代农业产业技术体系花生遗传育种岗位(SDAIT-04-02)
山东省农业科学院农业科技创新工程(CXGC2016A01)
作者简介
贾伟，女，主要从事花生抗逆生理研究。E-mail：17864272810@163．com.;李春娟，女，副研究员，主要从事花生品种资源及种质创新研究。E-mail：peanutlab@163．com。＂同为第一作＂;通讯作者：单世华，男，研究员，主要从事花生品种资源及种质创新研究。E-mail：shhshan@sina．com
