摘要
采用形态学特征结合rDNA-ITS序列比对方法,对陕西长安区草莓根腐病病原菌进行鉴定,以柯赫氏法则进行致病性验证,结果表明大双孢土赤壳(Ilyonectria macrodidyma)为该地区草莓根腐病的主要病原菌。生物学特性结果表明,该病原菌适应范围广,可在不同培养基上生长,菌丝最适生长温度为25℃,最适生长pH为4,分生孢子最适萌发温度和pH分别为30℃和7,分生孢子致死条件为45℃10min。
Root rot is a serious damage to the strawberry production at fruit stage in recent years. Chang＇an district of Xi＇an city is the main production area of strawberry in Shaanxi province in China, the causal pathogen fungus was identified as Ilyonectria macrodidyma based on the morphology char- acteristics, ribosomal DNA-ITS phylogenetic analysis and Koch＇s postulates pathogenicity test. The biological characteristics showed that the pathogen could grow under different culture media. The optimum temperature for mycelia growth was 25 ℃, perature and pH for spores germination were 30 ℃ ture was 45 ℃ for 10 min. and the suitable pH was pH 4. The optimum tem and pH 7 respectively. The conidia lethal tempera
作者
申光辉
薛泉宏
赵娟
SHEN Guanghui;XUE Quanhong;ZHAO Juan(College of Life Sciences,Northwest A＆F University,Yangling Shaanxi 712100,China;College of Natural Resources ＆ Environment,Northwest A＆F University,Yangling Shaanxi 712100,China;College of Food Science,Sichuan Agricultural University,Ya＇an Siehuan 625014,China;Institute of Plant and Environment Protection,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China)
出处
《西北农业学报》
CAS
CSCD
北大核心
2018年第7期1032-1040,共9页
Acta Agriculturae Boreali-occidentalis Sinica
基金
教育部长江学者和创新团队发展计划(IRT0748)
四川农业大学211工程双支计划(03572107)~~
作者简介
申光辉，男，博士研究生，讲师，研究方向为微生物资源与利用。E-mail：shenghuishen@163．com;通信作者：薛泉宏，男，教授，博士生导师，研究方向为微生物资源与利用。E-mail：xuequanhong@163．com
