摘要
为了确定一起山羊肺炎的病原体，从病羊肺脏中分离到1株巴氏杆菌样细菌，编号为ZY150911，对其进行形态学、生理生化和16SrDNA鉴定，并对其致病性和耐药性进行研究。分离株ZY150911经ATB-ID32E生化鉴定系统初步鉴定为Bibersteinia trehalosi。16SrDNA进化分析表明ZY150911与各B．trehalosi株形成同一进化支，与B．treh“zosi各株间的同源性为96．9％～98．4％，其中与模式菌株NCTC10370同源性为97．7％。根据形态学、生理生化和16SrDNA进化分析，将分离株ZY150911鉴定为B．trehalosi。致病性试验表明，分离株ZY150911对小鼠有强致病性，其LD50为3．2×10^6.2。分离株ZY150911对青霉素、氨苄西林耐药，对头孢噻吩、头孢呋辛、头孢曲松、庆大霉素、卡那霉素、阿米卡星、氧氟沙星、诺氟沙星、四环素、磺胺、氟苯尼考敏感。本研究在国内首次从动物体内分离到强致病性B．trehalosi，建议将其中文译名为海藻百伯坦菌，其可能为羊的一种新的致病菌，为羊B．trehalosi感染的诊断和流行病学研究奠定基础。
To diagnose a pathogen caused goat pneumonia, esigned as ZY150911,was isolated from the lung,and characterization, phylogenetic analysis of 16S rDNA, animal artificial infection, e bacteri tion, biologi SUS ceplibility of ZY150911 were studied. The strain ZY150911 was preliminarily identified as BiDer steinia trehalosi by API-ID32E biochemical identification system. Phylogenetic analysis indicated that strain ZY150911 clustered together with all 16 representative B. trehalosi strains to form one highly related branch and 16S rDNA nucleotide sequence shared 97.7% and 96.9%-98.4% similarity with NCTC10370 and the B. trehalosi strains, respectively. Our results confirmed that the strain ZY150911 belong to B. trehalosi. Animal artificial infection indicated that the strain ZY150911 was high pathogenic to mice and the 50% lethal dose （LD50） was 3.2x10^6.2 CFU. Anti biotics susceptibility test showed that the isolates was resistant to penicillin and ampicillin, and sensitive to cefalotin, cefuroxime, ceftriaxone, gentamicin, kanamycine, amikacin, ofloxacin, norloxacin,tetracycline,sulfadiazine and florfenicol. To our knowledge, this is the first report on the isolation of B. trehalosi from animal and with high pathogenic to mice. We suppose that the B. tre halosi may be a novel pathogen for goat. This study provid the base of diagnosis and epidemiologyof B. trehalosi infection in goat.
作者
李富祥
宋建领
赵文华
李华春
洪琼花
邵庆勇
杨仕标
LI Fu-xiang;SONG Jian-ling;ZHAO Wen-hua;LI Hua-chun;HONG Qiong-hua;SHAO Qing yong;YANG Shi-biao(Yunnan Animal Science and Veterinary Institute,Kunming 650224,China)
出处
《中国兽医学报》
CAS
CSCD
北大核心
2018年第8期1528-1532,共5页
Chinese Journal of Veterinary Science
基金
国家重点研发计划资助项目(SQ2017YFNC020122)
云南省重点新产品开发计划资助项目(2014BB014)
云南省现代农业奶牛产业技术体系建设资助项目(云财农[2009]171号)
云南省现代农业生猪产业技术体系建设资助项目(云财教[2013]160号)
云南省创新人才计划资助项目(2017HB090)
作者简介
李富祥（1979-），男，副研究员，硕士。;通讯作者,E-mail：yangsb3799@sina．com
