摘要
【目的】分离鉴定引起草鱼(Ctenopharyngodon idellus)肌肉糜烂的类志贺邻单胞菌(Plesiomonas shigeloide)菌株JX-09。【方法】从患病草鱼分离致病菌,经形态学观察、人工感染试验、生理生化特性测定和16S rRNA基因序列分析对其进行分类鉴定和致病性检验;同时对分离到的菌株做药物敏感性试验。【结果】从回归感染后的草鱼体内再次分离到菌株JX-09,表明该菌株为致病菌;菌株JX-09对草鱼的半致死量为6.4×104cfu/g。通过生化特征和分子系统学分析,将菌株JX-09鉴定为类志贺邻单胞菌。药敏试验表明该菌株对氨曲南、头孢唑林、头孢噻吩、头孢曲松等头孢类药物敏感,对卡那霉素、麦迪霉素、万古霉素和哌拉西林等耐药。【结论】类志贺邻单胞菌是草鱼肌肉糜烂病的致病菌,这是首次报道了该菌对草鱼有致病性。
[ Objective] The aim of this study is to identify strain JX-09 and confirmed that the strain is the pathogen of diseased grass carp （Ctenopharyngodon idellus）. [ Methods] A pathogenic strain JX-09 was isolated from the diseased grass carp. The strain was identified based on physiological and biochemical characteristics, and the sequence analysis of 16S rRNA. Virulence of strain JX-09 to healthy grass carp was also tested. Furthermore, drug sensitivity was detected with Kirby-Bauer＇ s agar diffusion method. [ Results ] Strain JX-09 was identified as Plesiomonas shigelloides by biochemical analysis and molecular biology. The P. shigelloides strain was re-isolated from the artificial infected grass carp, and the LDs0 was about 6.4 x lO^cfu/g. Drug sensitive tests showed that strain JX-09 was susceptible to aztreonam, cefazolin, cephalothin and ceftriaxone, and resistant to kanamycin, medicamycin, vancomycin and piperacillin. [ Conclusion ] Strain JX-09 was the pathogen of grass carp with muscle erosive disease. To the best of our knowledge, this is the first report that P. shigelloides as the pathogenic strain of grass carp.
出处
《微生物学报》
CAS
CSCD
北大核心
2014年第2期229-235,共7页
Acta Microbiologica Sinica
基金
现代产业技术体系建设经费“大宗淡水鱼类产业技术体系”(CARS-46)
国家科技支撑计划(2012BAD25B02)
广州市珠江科技新星专项(2012J2200078)~~
关键词
类志贺邻单胞菌
草鱼
鉴定
药敏试验
Plesiomonas shigeloides, Ctenopharyngodon idelhts, appraise, antibiotic sensitivity analysis
作者简介
胡钱东（1987-），男，四川达州人，硕士研究生，专业方向为水产动物疾病与免疫。E-mail：hqdl9871114@163．com
通信作者。Tel：＋86-20—81616813；E-mail：wushuqin001@21cn．com
