摘要
中药在提取炮制后残留大量生物质被简单堆弃,而其中的木质纤维素等生物质能被微生物有效利用降解,筛选到16株对木质纤维素有降解效果的菌株,对其中8株降解效果明显功能菌株进行了深入研究,经16(18)S rDNA鉴定发现5株细菌主要为Bacillus属、Streptomyces属和Enterobacter属,3株真菌为Ascomycete属、Aspergillus属和Trichosporon属;利用8株菌固相发酵中药废弃物,通过监测堆体温度、pH等参数,发现细菌类在发酵初期能迅速提高堆体温度,而真菌类发酵腐熟过程较缓慢,提示利用细菌-真菌联合组成降解菌系是提高中药废弃物固相发酵的重要手段。
Chinese medicinal herbal residues are simple to be discarded after the process of roasting,baking,and/or simmering,and the biomass such as lignocellulose can be effectively degraded by microbes. In this study,16 strains of strain with degradable effect on lignocellulose were screened. Among them 8 strains with significant degradable function adopting 16（ 18） S rDNA identification,five strains were found mainly to be the genera of Bacillus,Streptomyces and Enterobacter,and three fungi were the genera of Ascomycete,Aspergillus and Trichosporon. By monitoring the temperature of the reactor,p H and other parameters,it was found that the bacteria could rapidly increase the temperature of the reactor at the initial stage of fermentation,and the process of fungal fermentation was slower,suggested that a combination of bacteria-fungi degradable system is an important means to improve the solid phase fermentation of Chinese medicinal herbal residues.
作者
常帆
上官亦卿
吕睿
丁浩
贾凤安
CHANG Fan, SHANGGUAN Yi-qing, LYU Rui, DING Hao, JIA Feng-an(Microbial Metabolism Res. Ctr. , Shaanxi Pros. Inst. of Microbiol. , Xi＇an 71004)
出处
《微生物学杂志》
CAS
CSCD
2018年第3期64-70,共7页
Journal of Microbiology
基金
陕西省科学院青年联合创新项目(2016k-18)
陕西省社会发展科技攻关项目(2016SF-450)
陕西省科学院科技计划青年人才项目(2015K-21)
关键词
木质纤维素
中药废弃物
固相发酵
木质纤维素降解菌
lignoeellulose biomass
Chinese medicinal herbal residues
solid phase fermentation
lignocellulose degradable microbes
作者简介
常帆 男，助理研究员，博士。主要从事土壤环境和微生态研究工作。Tel：029—85350847，E—mail：fox387@163．com;通讯作者。女，助理研究员，硕士。主要从事微生物肥料及土壤改良技术研究工作。Tel：029—85350847，E—mail：290758979@qq．com
