摘要
为探究在镉(Cd)胁迫处理下锌(Zn)和硅(Si)对水稻中矿质元素从根到地上部转移的长期影响,以水稻耐镉基因型秀水110和镉敏感基因型HIPJ 1为供试材料,设置8个处理组合:3个Si处理水平(0,5,15μmol/L Na_2Si O_3·9H_2O)、3个Zn处理水平(0,1,10μmol/L Zn SO_4·7H_2O)和2个Cd处理水平(0,15μmol/L Cd Cl_2),测定其植株生物量、光合强度、抗氧化酶活性、脂质过氧化强度和矿质元素浓度。结果显示,Si(15μmol/L)或Zn(10μmol/L)可有效缓解Cd(15μmol/L)毒害对水稻植株生长的抑制效应,且以Zn的缓解效果更好。在Cd(15μmol/L)胁迫下添加Si(15μmol/L)或Zn(10μmol/L)可有效降低2个水稻基因型叶片中的丙二醛含量,并增强两者叶片中的过氧化氢酶活性,且以在秀水110中的效果更好。单一Zn或Si处理和Zn-Si复合处理均可减少HIPJ 1中的Cd^(2+)从根至地上部的转运。此外,Cd能显著降低水稻植株体内Ca、Mn、Mg等矿质元素的转运。单一Zn处理能够显著促进植物生长,且效果好于单一Si处理与Zn-Si联合处理。但是Zn-Si复合处理对提高Cd胁迫下矿质元素的吸收效果并不显著。
To investigate the long-term effects of zinc-silicon（Zn-Si）on mineral element translocation from root to shoot of rice under cadmium（Cd）stress,we performed this experiment using two rice genotypes（Xiushui110 and HIPJ 1）with eight combination treatments of three Si levels（0,5 and 15μmol/L Na2Si O3·9H2O）,three Zn levels（0,1 and 10μmol/L Zn SO4·7H2O）and two Cd levels（0 and 15μmol/L Cd Cl2）.Data on plant biomass,photosynthesis,antioxidant enzyme activity,lipid peroxidation and mineral element concentrations were analyzed.The combination treatments of Cd（15μmol/L）and Si（15μmol/L）or Zn（10μmol/L）resulted in higher shoot height and root length in both the genotypes as compared with the Cd（15μmol/L）treatment.Analysis of dry mass showed that the sole Zn alleviation was more effective on biomass increase than the sole Si in both the genotypes.The malondialdehyde（MDA）content in leaf dropped across all alleviation treatments in both the genotypes;Xiushui 110 had higher overall leaf catalase activity than HIPJ 1.The sole Zn and Si and the Zn-Si alleviation treatments reduced Cd translocation factor（TF）from root to shoot in HIPJ 1 but not in Xiushui 110.Analysis of TFs showed that Cd significantly reduced the movement of elements such as Ca,Mn and Mg.Zn alone treatment increased plant growth more than Si alone and Zn-Si treatments.However,Zn-Si treatment was not effective on improving mineral element uptake in Cd stressed plants.
出处
《浙江大学学报（农业与生命科学版）》
CAS
CSCD
北大核心
2018年第3期294-310,共17页
Journal of Zhejiang University:Agriculture and Life Sciences
基金
supported by the National Basic Research Program of China(973 Program)(517102-N51501/001)
National Natural Science Foundation of China--International(Regional) Cooperation and Exchange Program(517102-N11808ZJ)
Fundamental Research Funds for the Central
关键词
水稻
镉
锌
硅
基因型
毒性
rice （Oryza sativa）
cadmium
zinc
silicon
genotype
toxicity
作者简介
MAPODZEKE James Mutemachani （https：//orcid.org/0000-0002-4937-2329）, E-mail： jmapodzeke@yahoo.com;Corresponding author： SHAMSI Imran Haider （https：//orcid.org/0000-0002-8545-660X）, E-mail： drimran@zju.edu.cn
