摘要
目的建立用超高效液相色谱法(ultra performance liquid chromatography,UPLC)同时测定饮料中的山梨酸、苯甲酸、脱氢乙酸、糖精钠和安赛蜜的分析方法。方法样品中山梨酸、苯甲酸、脱氢乙酸、糖精钠和安赛蜜用水提取,经BEH C_(18)柱(2.1 mm×100 mm,1.7μm)分离,在波长225 nm处检测。结果此方法的线性范围是5~100μg/mL,回收率为95.5%~100.2%,相对标准偏差(relative standard deviation,RSD)为1.0%~3.1%。结论此方法简便快速,准确可靠,灵敏度高,可用于同时检测饮料中的山梨酸、苯甲酸、脱氢乙酸、糖精钠和安赛蜜。
Objective To establish a method for simultaneous determination of sorbic acid, benzoic acid, dehydroacetic acid, saccharin sodium and acesulfame in beverages by ultra performance liquid chromatography （UPLC）. Methods Sorbic acid, benzoic acid, dehydroacetic acid, saccharin sodium and acesulfame in samples were extracted in water, and then were separated through B EH C18 （2.1 mmx 100 mm, 1.7 μm） and detected at a wavelength of 225 nm. Results The linear range of this method was 5-100 μg/mL, and The recovery rates were 95.5%-100.2% with relative deviation standard （RSD） of 1.0%-3.1%. Conclusion This method is simple, rapid, accurate, reliable and sensitive, which can be used for simultaneous detection of sorbic acid, benzoic acid, dehydroacetic acid, sodium saccharin and acesulfame in beverages.
作者
张剑峰
孙艳芳
王缘
孙海会
ZHANG Jian-Feng;SUN Yan-Fang;WANG Yuan;SUN Hai-Hui(Heilongjiang Province Center for Disease Control and Prevention,Harbin 150030,China)
出处
《食品安全质量检测学报》
CAS
2018年第14期3801-3806,共6页
Journal of Food Safety and Quality
关键词
饮料
山梨酸
苯甲酸
脱氢乙酸
糖精钠
安赛蜜
超高效液相色谱法
beverages
sorbic acid
benzoic acid
dehydroacetic acid
sodium saccharin
acesulfame
ultra performance liquid chromatography
作者简介
张剑峰，硕士，主任技师，主要研究方向为食品安全、卫生检验。E-mail：13069887711@163．com。;通讯作者：孙艳芳，副主任技师，主要研究方向为食品安全，卫生检验。E-mail：fangzi_2011@163．com
