摘要
目的:在确保化合物稳定性的前提下,评价乙酰化鼠尾草酸对小鼠海马神经元的保护作用。方法:C57小鼠胎鼠海马神经元经乙酰化鼠尾草酸5、20、80μmol/L预处理后,加入H_2O_2,抗体染色,观察细胞状态并评估神经元的活性。同时,高效液相色谱法测定168 h内四种不同保存条件下乙酰化鼠尾草酸的含量。结果:乙酰鼠尾草酸预处理组与模型组相比,小鼠海马神经元活性增强,LDH的漏出量下降,细胞内MDA产生下降,SOD活性升高,caspase-3 mRNA的表达降低;在168 h内,乙酰鼠尾草酸在四种不同溶液保存条件下,含量稳定。结论:乙酰鼠尾草酸在不同溶液中结构性质稳定,对小鼠海马神经元的损伤具有良好的保护作用。
Objective： To evaluate the effect of acetylated carnosic acid on hippocampal neurons. Methods： Neurons were treated with acetylated carnosic acid at concentrations of 5,20,80μmol/L for 30 min prior to H_2O_2（50. 0 μmol/L） treatment. After 6 h,the morphology and activity of neurons were evaluated. Meanwhile,contents of acetylated carnosic acid were detected by HPLC in 0 ~ 168 h under different storage conditions. Results： The activity of hippocampal neurons increased after the pretreatment with acetylated carnosic acid. The content of acetylated carnosic acid kept stable. Conclusion： Acetylated carnosic acid can protect injured hippocampal neurons induced by H_2O_2,and the compound keeps stability well.
作者
刘佳
于浩飞
张荣平
胡炜彦
魏静
姜晓楠
张勇
张兰春
Liu Jia;Yu Haofei;Zhang Rongping;Hu Weiyan;Wei Jing;Jiang Xiaonan;Zhang Yong;Zhang Lanchun(School of Pharmaceutical Sciences and Yunnan Key Laboratory of Pharmacology for Natural Products,No.1 School of Clinical Medicine,Kunming Medical University,Ktmming 650500;Hai Yuan School of Kunming Medical College,Kunming 650101;Yunnan Lufeng County second people~ Hospital,Chuxiong 651200;Kunming Pharmaceutical Co.Ltd.Kunming 650000)
出处
《中药药理与临床》
CAS
CSCD
北大核心
2018年第3期30-32,共3页
Pharmacology and Clinics of Chinese Materia Medica
基金
科技部十二五支撑计划(2014BAI01B01~04)
云南省自然基金-昆医专项(2015FB006,2017FE467(-024))
云南省天然药物药理重点实验室(2014G003)
作者简介
通讯作者:张兰春
