摘要
目的:利用生物信息学对1型糖尿病小鼠心肌组织中微小RNA(miR)-142-3p进行长链非编码RNA(lncRNA)和环状RNA(circRNA)靶标预测。方法:22只C57小鼠随机分为糖尿病模型组(n=15)和对照组(n=7),糖尿病模型组小鼠经腹腔一次性注射链脲佐菌素(STZ)建立1型糖尿病小鼠模型,对照组注射柠檬酸缓冲液。建模8周后终止实验,检测左室质量指数(LVWI)和心脏功能,HE染色结合定量分析软件检测心肌细胞大小;通过miRNA表达谱芯片技术甄别miRNAs的差异性,qRT-PCR确定差异表达;采用微小RNA靶标分析软件miRanda和TargetScan进行靶标预测,对预测到的circRNA宿主基因(host gene)进行基因功能聚类解析。结果:(1)糖尿病模型组心肌组织中miR-142-3p表达显著降低;(2)在高严谨条件下,用miRanda和TargetScan能够同时预测到miR-142-3p的靶标lncRNA有35个、circRNA有14个;(3)宿主基因功能预测显示miR-142-3p参与糖代谢相关的信号通路。结论:miR-142-3p可能通过调控部分lncRNA和circRNA,参与糖尿病心肌病的发生。
Objective：To predict new long non-coding RNA（lncRNA）and circular RNA（circRNA）targeting miR-142-3 p in type 1 diabetic mice myocardium by bioinformatics. Methods：A total of 22 C57 mice were randomly divided into type 1 diabetes group（n=15）and control group（n=7）.Type 1 diabetes mice were modeled by once intraperitoneal injection of streptozotocin,while the control mice were injected with citric acid buffer.Eight weeks after modeling,the left ventricular mass index（LVWI）and cell morphology of the myocardium and the quantitative analysis of cell area were detected respectively by Echocardiography and HE staining.Target mapping was performed with miRanda and TargetScan analysis software.Genetic function clustering was performed by GO and KEGG analysis. Results：MiR-142-3 p expression was decreased in type 1 diabetic mice hearts.The prediction of 35 lncRNAs and14 circRNAs was found by miRanda and TargetScan under high stringency conditions.Furthermore,the signaling pathways related to sugar metabolism were disclosed by GO and KEGG analysis. Conclusions：MiR-142-3 p may be involved in the development of diabetic cardiomyopathy by regulating certain lncRNAs and circRNAs.
作者
孟哲颖
胡兵
陈翠
曹洪丽
王静怡
申锷
MENG Zheying;HU Bing;CHEN Cui;CAO Hongli;WANG Jingyi;SHEN E(Department of Ultrasound in Medicine,Shanghai Jiao Tong University Affiliated Sixth People＇s Hospital,Shanghai Institute of Ultrasound in Medicine,Shanghai 200233;Department of Ultrasound in Medicine,Tongren Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200366,China)
出处
《国际心血管病杂志》
2018年第4期214-218,共5页
International Journal of Cardiovascular Disease
基金
国家自然科学基金(81270208)
上海市长宁区科学技术委员会重点项目(CNKW2017Z03)
作者简介
通信作者：申锷,Email：shene2008＠163.com
