摘要
将72只SD大鼠随机均分为空白对照组、阳性对照组(8–甲氧基补骨脂素、蒽)和白芷(生药)低、中、高3个剂量组(0.93、4.65、9.3 g/kg)并设立有/无紫外辐射2种条件,每处理6只,采用Draize法测定不同时间点动物皮肤刺激反应积分并耳廓打孔称重评价皮肤光毒性;运用q PCR方法对各组大鼠皮肤组织炎症相关的环氧合酶–2(COX–2)、前列腺素E2受体1(EP1)、肿瘤坏死因子–α(TNF–α)、白细胞介素–1β(IL–1β)和白细胞介素–6(IL–6)5个基因的m RNA表达量进行比较分析。结果表明:在无紫外辐照情况下,各组大鼠皮肤均没有出现红斑和水肿现象,皮肤刺激反应积分都为0;在辐射条件下,空白对照组大鼠皮肤也没有出现红斑和水肿现象,白芷水提物只在中、高剂量条件下部分大鼠皮肤出现轻微的红斑甚至水肿现象;相比空白对照组,只有阳性对照组大鼠的耳片质量显著增加(P<0.05);5个炎症相关基因的表达量结果显示,白芷水提物只在高剂量条件下大鼠皮肤组织IL–6m RNA的相对表达量显著上升(P<0.05)。综上表明,口服推荐剂量(0.93 g/kg)白芷水提物对大鼠皮肤无光毒性反应,符合食品安全性要求。
72 SD rats were randomly divided into five groups： blank control group, positive control groups （8-methoxypsoralen and anthracene） and low, medium and high dose groups of radix Angelicae dahurica and each group was treated by the two conditions with or without ultraviolet radiation. The skin irritation reaction score at different time points was measured using Draize method, and the skin phototoxicity was evaluated by weight change of rat ear slices. The mRNA expression levels of five inflammatory related genes （COX-2, EP1, TNF-α, IL-1β and IL-6） in skin tissue of each group were analyzed and compared by qPCR method. Result： without ultraviolet radiation, there were no erythema and edema in the skin of rats in each group, and the skin irritation reaction score was 0. With the radiation, the rats in control group did not appear skin erythema and edema; some rats appear slight erythema or edema phenomenon only in the medium or/and high doses of radix Angelicae dahurica. In addition, compared with the blank control group, the ear weight of the rats only in the positive control group increased significantly （P〈0.05）, and the expression levels of five inflammation related genes showed that the relative expression level of only IL-6 mRNA in skin tissue of rats with the high dose of Angelica dahurica water extract increased significantly（P〈0.05）. These results indicated that the recommended dose of radix Angelicae dahurica water extract has no phototoxic effects on the skin of rats and meets the requirements of food safety.
作者
刘硕
黄霞
林勇
林海燕
汪启明
刘仲华
LIU Shuo;HUANG Xia;LIN Yong;LIN Haiyan;WANG Qiming;LIU Zhonghua(Hunan Co-Innovation Center for Utilization of Botanical Functional Ingredients,Changsha,Hunan 410128,China;Infinitus（China）Company Ltd.,Guangzhou,Guangdong 510623,China;a.College of Resources and Environment,b.College of Bioscience and Biotechnology,Hunan Agricultural University,Changsha,Hunan 410128,China)
出处
《湖南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2018年第4期424-429,共6页
Journal of Hunan Agricultural University(Natural Sciences)
基金
湖南省自然科学基金项目(2017JJ3106)
作物种质创新与资源利用重点实验室开放研究项目(15KFXM05)
关键词
大鼠
白芷水提物
紫外辐射
皮肤光毒性
rats
radix Angelicae dahuricae water extract
ultraviolet （UV） radiation
skin phototoxicity
作者简介
刘硕（1982-），男，湖南长沙人，硕士，工程师，主要从事植物功能成分化学及高效利用研究，370516021@qq.com；;通信作者，林勇，博士，副教授，主要从事植物功能成分化学与生物活性研究，ly2005306@163.com
