摘要
建立强力枇杷露中齐墩果酸和熊果酸含量测定方法。采用HPLC法,色谱柱为Sepax Aurethyst C18-H(4.6mm×250 mm,5μm);流动相为乙腈-甲醇-0.5%乙酸铵溶液(体积比67∶12∶21);流量为1.0 m L/min;柱温为30℃;检测波长为210nm。齐墩果酸的进样量在0.05~1.59μg呈良好的线性关系(r=1.0000),精密度RSD=1.23%,平均加样回收率为93.7%,RSD=1.40%;熊果酸的进样量在0.19~6.20μg呈良好的线性关系(r=1.000 0),精密度RSD=0.20%,平均加样回收率为98.6%,RSD=1.96%。该方法简便、准确、重复性好,可用于强力枇杷露中齐墩果酸和熊果酸的含量测定。
To establish a method for determining oleanolic acid and ursolic acid in Qiangli Pipa syrup. A HPLC method was adopted. A Sepax Aurethyst C18-H column（4.6 mm ×250 mm, 5 μm）was used with the column temperature of 30 ℃. The mobile phase consisted of acetonitrile-methanol-0.5% ammonium acetate（ν ∶ν ∶ν,67 ∶12 ∶21）. The flow rate was 1.0 m L/min, detective wavelength was set at 210 nm. The linear range of oleanolic acid was 0.05-1.59 μg（r=1.000 0）, the precision（RSD） was 1.23%, the average recovery was 93.7%, with RSD=1.40%. The linear range of ursolic acid was 0.19-6.20 μg（r=1.000 0）, the precision（RSD） was 0.20%, the average recovery was 98.6%, with RSD =1.96%. The method is simple, accurate, repeatable and suitable for the determination of oleanolic acid and ursolic acid in Qiangli Pipa syrup.
作者
袁海英
叶利明
YUAN Haiying1,2 , YE Liming1(1. West China School of Pharmacy,Sichuan University,Chengdu 610041, China; 2. China Resources Sanjiu（Ya＇an） pharmaceutical Co., Ltd.,Ya＇an 625000,China)
出处
《中国测试》
CAS
北大核心
2018年第5期63-66,共4页
China Measurement & Test
作者简介
袁海英（1975-）,女,四川成都市人,执业中药师,硕士研究生,专业方向为药物分析.;通信作者：叶利明（1963-）,男,四川成都市人,教授,硕士生导师,博士,主要从事中药、中药材及化学药质量标准研究.
