摘要
目的采用超高效液质联用方法测定多潘立酮口腔崩解片的含量。方法色谱柱:ACQUITY UPLC BEH C_(18)(2.1 mm×50 mm,1.7μm),流动相:甲醇-2 mmol/L乙酸铵水溶液(80∶20),流速:200μL/min;柱温:35℃,质谱采用多离子反应监测模式(MRM),选择正离子模式检测。结果多潘立酮在0.006 2~6.2μg/mL范围内与峰面积呈良好的线性关系(r=0.996 3,n=6),最低定量限为6.2 ng/mL。加样回收率为100.23%(n=6),RSD为1.78%。结论本方法灵敏度高、专属性强、结果准确,可用于多潘立酮口腔崩解片的质量控制。
Objective To establish a UPLC-MS/MS method for the determination of domperidone orally-tablets.Methods The experiment was performed with an ACQUITY UPLC BEH C18 column（2.1 mm ×50 mm,1.7 μm） at 35 ℃.The mobile phase consisted of methanol-2 mmol/L ammonium acetate（80∶ 20）,and the flow rate was 200 μL/min.The sample was quantified using LC-MS/MS in the multiple reaction monitoring（MRM）system under ESI positive mode.Results The calibration curve was linear in the range from 0.006 2 to 6.2 μg/mL with r = 0.996 3,the minimum quantitation limit being 6.2 ng/mL.The sample recovery was 100.23%（n = 6） with the RSD being 1.78%.Conclusion The method presents high sensitivity,specificity and accuracy.Therefore,it can be used for the quality control of domperidone orally disintegrating tablets.
作者
谢君
吕玲燕
冯星月
蒋志涛
余辉
陈晓峰
XIE Jun;LU Ling-yan;FENG Xing-yue;JIANG Zhi-tao;YU Hui;CHEN Xiao-feng(Zhangjiagang Hospital of Traditional Chinese Medicine,Affiliated Hospital of Nanjing University of Traditional Chinese Medicine,Graduate Workstation of Enterprises in Jiangsu Province, Zhangjiagang 215600, China)
出处
《实用药物与临床》
CAS
2018年第3期302-304,共3页
Practical Pharmacy and Clinical Remedies
基金
苏州市科技发展计划项目(SYSD2016161)
关键词
多潘立酮
液质联用
含量测定
Domperidone
UPLC-MS/MS
Content determination
作者简介
通讯作者:陈晓峰
