摘要
目的:观察光刺激对3x Tg阿尔茨海默病(AD)模型小鼠海马长时程增强(LTP)及β淀粉样蛋白(Aβ)的影响。方法:4～6月3x Tg AD小鼠20只随机分为光刺激(PS)组,未刺激(NS)组,同背景野生型小鼠10只为对照组。PS组小鼠接受2 Hz光刺激4周,其余2组正常环境饲养。4周后断头取脑,制作离体脑片,诱导海马LTP,并检测小鼠海马组织内Aβ蛋白含量。结果:NS组小鼠LTP明显受抑,海马组织内Aβ含量较对照组明显升高(P<0.05)。PS组小鼠较NS组小鼠海马Aβ蛋白水平明显降低(P<0.05),LTP明显增强(P<0.05)。结论:光刺激增强3x Tg AD模型小鼠海马LTP,降低海马Aβ含量。
Objective： To observe the effect ofphotic stimulation on long term potentiation （LTP） and A beta in the hippocampus of 3xTg Alzheimer＇s model mice. Methods： Twenty 4 - 6 month 3xTg AD mice were random- ly divided into photic stimulation （PS） group and unstimulated （NS） group. Ten wild type mice in the same back- ground were in the control group. AD mice in the PS group received 2 Hz photic stimulation for 4 weeks. After the mice were decapitated, the in vitro hippocampal LTP was induced and the A beta protein content in the hippo- campus of mice was detected. Results： The LTP of mice in NS group was significantly inhibited, and the content of A beta in the hippocampus was significantly higher than that in control group （both P〈0.05）. The level of A beta protein in hippocampus of mice in PS group was significantly lower than that in NS group, while LTP in- creased significantly （both P〈0.05）. Conclusion： Photic stimulation enhanced the hippocampal LTP and reduced the content of A beta in 3xTg AD mice model.
出处
《神经损伤与功能重建》
2018年第2期55-56,61,共3页
Neural Injury and Functional Reconstruction
基金
国家自然科学基金(No.81671064
No.81371222)
日本文部科学省科学研究费补助金(No.22500360)
关键词
阿尔茨海默病
光刺激
长时程增强
Β淀粉样蛋白
Alzheimer＇s Disease
photic stimulation
long term potentiation
β-amyloid protein
作者简介
通讯作者：王芙蓉wangfurong．china@163．com
