摘要
目的动态观察皮肤再生医疗技术(moist exposed burn therapy/moist exposed bum ointment,MEBT/MEBO)对大鼠慢性难愈合创面组织中VEGFR-2表达水平的影响,探讨MEBT/MEBO促进创面愈合的分子学机制。方法按照随机数表法将90只健康成年SPF级SD雄性大鼠随机分为空白组(18只)、对照组(18只)、模型组(18只)、MEBO组(18只)和贝复济组(18只),其中空白组仅做备皮处理,对照组做全层皮肤缺损创面模型,模型组、MEBO组和贝复济组做慢性难愈合创面模型;模型建立后,空白组大鼠备皮后皮肤予以生理盐水纱布湿敷,对照组及模型组大鼠创面予以生理盐水纱布覆盖治疗,MEBO组大鼠创面予以湿润烧伤膏(moist exposed burn ointment,MEBO)药纱覆盖治疗,贝复济组大鼠创面予以贝复济药纱覆盖治疗,并分别于治疗后第3、7、14天检测、对比各组大鼠创面组织中VEGFR-2蛋白及VEGFR-2 mRNA的表达水平。结果创面处理后第3、7、14天,空白组大鼠皮肤组织中VEGFR-2蛋白及VEGFR-2 mRNA表达水平均无明显变化,P均>0.05,差异无统计学意义;而对照组、模型组、MEBO组及贝复济组大鼠创面组织中VEGFR-2蛋白及VEGFR-2 mRNA表达水平变化均较明显,P均<0.05,差异具有统计学意义,其中对照组、MEBO组及贝复济组大鼠创面组织中VEGFR-2蛋白及VEGFR-2 mRNA表达水平均呈先升高后降低的趋势,而模型组则呈递增趋势。治疗第3、7、14天,MEBO组大鼠创面组织中VEGFR-2蛋白和VEGFR-2 mRNA表达水平均高于模型组,P均<0.05.差异具有统计学意义。结论 MEBT/MEBO可通过上调创面组织中VEGFR-2的表达水平,促进创面愈合。
Objective To have a dynamic observation of the influence of MEBT/MEBO on the expression level of VEGFR-2 in rat chronic and hard-to-healing wound tissues and explore the molecular mechanism of MEBT/MEBO promoting wound healing. Methods 90 healthy SPF SD male rats were randomly divided into five groups： blank group（ 18）, control group（18）, model group（18）, MEBO group（18）, BFGF group（18）. For blank group, only skin preparation was performed; for control group, the full thickness skin defect model was established; for model group, MEBO group and BFGF group, chronic and hard-to-healing wound models were established. After the models were established, the prepared skin of rats in blank group was dressed with normal saline gauze; rats＇ wounds of control group and model group were covered with normal saline gauze; rats＇ wounds of MEBO group were treated with MEBO gauze; rats＇ wounds of BFGF group were treated with BFGF gauze. The expression level of protein VEGFR-2 and VEGFR-2 mRNA in rats＇ wounds of the above groups were determined and compared after 3, 7 and 14 days of treatment respectively. Results On day 3,day 7 and day 14 after wound management, no significant change was observed in the expression level of protein VEGFR-2 and VEGFR-2 mRNA in rats＇ skin tissues of blank group（P 0. 05, no statistically significant difference）. Significant changes were observed in the expression level of protein VEGFR-2 and VEGFR-2 mRNA in rats＇ wound tissues of control group, model group, MEBO group and BFGF group and the difference was statistically significant（P 0. 05）. In control group, MEBO group and BFGF group, expression level of protein VEGFR-2 and VECFR-2 mRNA increased at first and decreased after a period of time,while it kept increasing all the time in model group. On days 3,7 and 14 of treatment, expression level of protein VEGFR-2 and VEGFR-2 mRNA in rats＇ wounds of MEBO group was higher than model group and the comparison showed statistically significant difference（P0. 05）. Conclusion MEBT/MEBO can increase the expression level of VEGFR-2 in wound tissues, hence promoting wound healing.
出处
《中国烧伤创疡杂志》
2017年第6期399-405,共7页
The Chinese Journal of Burns Wounds & Surface Ulcers
基金
2017年中国红十字基金会徐荣祥再生命公益基金科研项目(项目编号:中国红基会[2017]188号)
国家自然科学基金项目(项目编号:81560776)~~
关键词
慢性难愈合创面
皮肤再生医疗技术
湿润烧伤膏
VEGFR-2
动态调控
实验研究
Chronic and hard-to-healing wounds
Skin regenerative medical technology
Moist exposed burn oint-ment
VEGFR-2
Dynamic regulation
Experimental research
作者简介
唐乾利，E—mail：htmgx@163．com
